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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
10763 | 5' | -51.6 | NC_002794.1 | + | 118048 | 0.66 | 0.991528 |
Target: 5'- cGAGCGUgGCCCGUCucgcggaAGACGgccgACg--- -3' miRNA: 3'- -CUCGCAaCGGGCAG-------UCUGUa---UGaaca -5' |
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10763 | 5' | -51.6 | NC_002794.1 | + | 79354 | 0.67 | 0.989109 |
Target: 5'- cGGCGcagaGCCUGUCGGACAcGCUg-- -3' miRNA: 3'- cUCGCaa--CGGGCAGUCUGUaUGAaca -5' |
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10763 | 5' | -51.6 | NC_002794.1 | + | 104031 | 0.87 | 0.208806 |
Target: 5'- cGAGCGUU-CgCCGUCAGACAUACUUGUc -3' miRNA: 3'- -CUCGCAAcG-GGCAGUCUGUAUGAACA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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