Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1077 | 3' | -55.5 | NC_000929.1 | + | 22688 | 0.66 | 0.544115 |
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Target: 5'- -gGCaacgUCA-GCGuCUGGAGUGGaCGCg -3' miRNA: 3'- aaCGgua-AGUcCGC-GACCUUACC-GCG- -5' |
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| 1077 | 3' | -55.5 | NC_000929.1 | + | 27370 | 0.67 | 0.457112 |
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Target: 5'- cUGCCAUgcugggggcGGCGCUGGAua-GCGUc -3' miRNA: 3'- aACGGUAagu------CCGCGACCUuacCGCG- -5' |
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| 1077 | 3' | -55.5 | NC_000929.1 | + | 25742 | 0.68 | 0.416479 |
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Target: 5'- gUGCCG---GGGCGCUGGGcuggAUGGgGa -3' miRNA: 3'- aACGGUaagUCCGCGACCU----UACCgCg -5' |
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| 1077 | 3' | -55.5 | NC_000929.1 | + | 22594 | 0.69 | 0.342229 |
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Target: 5'- gUUGCCAgcCAGGCGCUuuccaucGAcccGGCGCg -3' miRNA: 3'- -AACGGUaaGUCCGCGAc------CUua-CCGCG- -5' |
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| 1077 | 3' | -55.5 | NC_000929.1 | + | 22058 | 0.69 | 0.333647 |
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Target: 5'- cUGUCGcagCAGGUGCaacgGGAGcGGCGCu -3' miRNA: 3'- aACGGUaa-GUCCGCGa---CCUUaCCGCG- -5' |
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| 1077 | 3' | -55.5 | NC_000929.1 | + | 24313 | 0.72 | 0.217673 |
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Target: 5'- -aGCCG---GGGCGCUGGGuaucgcUGGCGUg -3' miRNA: 3'- aaCGGUaagUCCGCGACCUu-----ACCGCG- -5' |
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| 1077 | 3' | -55.5 | NC_000929.1 | + | 29647 | 0.8 | 0.061553 |
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Target: 5'- aUGCCGaagAGGCGCUGGAAUGGCu- -3' miRNA: 3'- aACGGUaagUCCGCGACCUUACCGcg -5' |
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| 1077 | 3' | -55.5 | NC_000929.1 | + | 14786 | 1.1 | 0.000355 |
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Target: 5'- cUUGCCAUUCAGGCGCUGGAAUGGCGCa -3' miRNA: 3'- -AACGGUAAGUCCGCGACCUUACCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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