Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10772 | 3' | -57 | NC_002794.1 | + | 93341 | 0.66 | 0.903075 |
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Target: 5'- gGCCUGAgcACCaaGCUGGuGCC-GCAGa -3' miRNA: 3'- -UGGACUacUGGagCGACU-CGGaCGUC- -5' |
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| 10772 | 3' | -57 | NC_002794.1 | + | 58634 | 0.66 | 0.890168 |
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Target: 5'- cGCCUGAUcGCCacgcaGCUGGGCgacgggcugCUGCAGc -3' miRNA: 3'- -UGGACUAcUGGag---CGACUCG---------GACGUC- -5' |
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| 10772 | 3' | -57 | NC_002794.1 | + | 40201 | 0.66 | 0.883379 |
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Target: 5'- uCCUGugGUGACagCUCGCggucgaGAGCCUGCc- -3' miRNA: 3'- uGGAC--UACUG--GAGCGa-----CUCGGACGuc -5' |
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| 10772 | 3' | -57 | NC_002794.1 | + | 123500 | 0.68 | 0.821665 |
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Target: 5'- gACCUG-UGcaucGCCggGCUGccGGCCUGCAGc -3' miRNA: 3'- -UGGACuAC----UGGagCGAC--UCGGACGUC- -5' |
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| 10772 | 3' | -57 | NC_002794.1 | + | 79329 | 0.68 | 0.775561 |
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Target: 5'- uACCUGAgucagGGCCgcccgaucgcggCGCaGAGCCUGuCGGa -3' miRNA: 3'- -UGGACUa----CUGGa-----------GCGaCUCGGAC-GUC- -5' |
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| 10772 | 3' | -57 | NC_002794.1 | + | 77655 | 0.69 | 0.729967 |
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Target: 5'- cACCUGugcGGCC-CGC-GGGUCUGCGGg -3' miRNA: 3'- -UGGACua-CUGGaGCGaCUCGGACGUC- -5' |
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| 10772 | 3' | -57 | NC_002794.1 | + | 68333 | 0.71 | 0.654258 |
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Target: 5'- gGCCUGGUGACCggGUUcauccgccaagagacGGGCCUGCuGa -3' miRNA: 3'- -UGGACUACUGGagCGA---------------CUCGGACGuC- -5' |
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| 10772 | 3' | -57 | NC_002794.1 | + | 95623 | 1.08 | 0.003149 |
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Target: 5'- gACCUGAUGACCUCGCUGAGCCUGCAGu -3' miRNA: 3'- -UGGACUACUGGAGCGACUCGGACGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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