miRNA: 3'- gGUUCa----CGUCGu---UC-UCGCACCuCAAG- -5'
miRNA: 3'- -GGUUCACGUcGUUCU---CGCAccUCAAG- -5'
miRNA: 3'- -GGUucACGUCgUUCUCGCACCuCaAG- -5'
Results 21 - 23 of 23 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10774 | 3' | -51.7 | NC_002794.1 | + | 86986 | 0.66 | 0.993167 |
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Target: 5'- aCGAGguccgGCGGCuuucGGcGGCGUGG-GUUCc -3' miRNA: 3'- gGUUCa----CGUCGu---UC-UCGCACCuCAAG- -5' |
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| 10774 | 3' | -51.7 | NC_002794.1 | + | 98430 | 0.66 | 0.994081 |
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Target: 5'- uCCAGGUGCAaCGGGAcauGCGUa-AGUUCg -3' miRNA: 3'- -GGUUCACGUcGUUCU---CGCAccUCAAG- -5' |
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| 10774 | 3' | -51.7 | NC_002794.1 | + | 138106 | 0.66 | 0.994081 |
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Target: 5'- aCCGucUGCAG-GAGGGCGUGGuGcUCg -3' miRNA: 3'- -GGUucACGUCgUUCUCGCACCuCaAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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