Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10775 | 3' | -60.8 | NC_002794.1 | + | 126389 | 0.7 | 0.463303 |
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Target: 5'- gGGGCCUGCgcggcggcgUGCGCggcggucgcgaucUGGCUGCUGg-- -3' miRNA: 3'- gCUCGGACG---------ACGCG-------------ACCGACGACguc -5' |
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| 10775 | 3' | -60.8 | NC_002794.1 | + | 58454 | 0.72 | 0.37088 |
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Target: 5'- --cGCCUgGCcGCGCUGGC-GCUGCAc -3' miRNA: 3'- gcuCGGA-CGaCGCGACCGaCGACGUc -5' |
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| 10775 | 3' | -60.8 | NC_002794.1 | + | 56102 | 0.72 | 0.363052 |
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Target: 5'- aCGuGCgUGCUGCGCUGGCccgucaugacggUGaaCUGCAGc -3' miRNA: 3'- -GCuCGgACGACGCGACCG------------AC--GACGUC- -5' |
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| 10775 | 3' | -60.8 | NC_002794.1 | + | 147671 | 0.72 | 0.363052 |
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Target: 5'- gGAGCCUGCUcGCGCUcgaggacccGGCgGCgaUGCGGc -3' miRNA: 3'- gCUCGGACGA-CGCGA---------CCGaCG--ACGUC- -5' |
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| 10775 | 3' | -60.8 | NC_002794.1 | + | 58634 | 0.72 | 0.35534 |
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Target: 5'- --cGCCUgaucgccacgcaGCUGgGCgacgGGCUGCUGCAGc -3' miRNA: 3'- gcuCGGA------------CGACgCGa---CCGACGACGUC- -5' |
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| 10775 | 3' | -60.8 | NC_002794.1 | + | 124528 | 0.73 | 0.347743 |
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Target: 5'- gGAGCC-GCcGaCGCUGGC-GCUGCGGc -3' miRNA: 3'- gCUCGGaCGaC-GCGACCGaCGACGUC- -5' |
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| 10775 | 3' | -60.8 | NC_002794.1 | + | 125973 | 0.73 | 0.340264 |
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Target: 5'- cCGAGuuCCUGCUGCaGCUGGCcacGCaGCAGc -3' miRNA: 3'- -GCUC--GGACGACG-CGACCGa--CGaCGUC- -5' |
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| 10775 | 3' | -60.8 | NC_002794.1 | + | 92721 | 0.97 | 0.007775 |
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Target: 5'- uCGAGCCUGUcGCGCUGGCUGCUGCAGc -3' miRNA: 3'- -GCUCGGACGaCGCGACCGACGACGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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