Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10782 | 3' | -48.6 | NC_002794.1 | + | 152822 | 0.66 | 0.999324 |
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Target: 5'- gGUGCCGUGCggacacGGcGUcAGCgacGCCUACg -3' miRNA: 3'- -UAUGGCAUGa-----UC-CAuUCGa--UGGAUGa -5' |
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| 10782 | 3' | -48.6 | NC_002794.1 | + | 149704 | 0.67 | 0.997809 |
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Target: 5'- -gGCCGccugGC-GGGUGGGCcGCCUGCc -3' miRNA: 3'- uaUGGCa---UGaUCCAUUCGaUGGAUGa -5' |
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| 10782 | 3' | -48.6 | NC_002794.1 | + | 68503 | 0.69 | 0.994128 |
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Target: 5'- -gGCCGUGC-GGGUGAuCUGCCaGCg -3' miRNA: 3'- uaUGGCAUGaUCCAUUcGAUGGaUGa -5' |
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| 10782 | 3' | -48.6 | NC_002794.1 | + | 125869 | 0.69 | 0.993191 |
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Target: 5'- -gGCCGUG-UGGGaGAGCUGCCU-Cg -3' miRNA: 3'- uaUGGCAUgAUCCaUUCGAUGGAuGa -5' |
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| 10782 | 3' | -48.6 | NC_002794.1 | + | 55560 | 0.71 | 0.97312 |
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Target: 5'- uUGCCGUACUGcacGGgcAGCcgaaaguagagUACCUGCUu -3' miRNA: 3'- uAUGGCAUGAU---CCauUCG-----------AUGGAUGA- -5' |
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| 10782 | 3' | -48.6 | NC_002794.1 | + | 87720 | 1.07 | 0.021369 |
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Target: 5'- cAUACCGUACUAGGUAAGCUACCUACUa -3' miRNA: 3'- -UAUGGCAUGAUCCAUUCGAUGGAUGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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