Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
10782 | 5' | -48.1 | NC_002794.1 | + | 20655 | 0.66 | 0.999689 |
Target: 5'- cGAGCAAGGgg----UCCUGCUGCu- -3' miRNA: 3'- cUUCGUUCCauacgaAGGAUGAUGca -5' |
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10782 | 5' | -48.1 | NC_002794.1 | + | 58705 | 0.67 | 0.999063 |
Target: 5'- cGGGCu-GGUGcGCUUCCUGCaccGCGUc -3' miRNA: 3'- cUUCGuuCCAUaCGAAGGAUGa--UGCA- -5' |
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10782 | 5' | -48.1 | NC_002794.1 | + | 91304 | 0.72 | 0.971276 |
Target: 5'- uGAGCGAGGc--GCUcUUCUACUACGUg -3' miRNA: 3'- cUUCGUUCCauaCGA-AGGAUGAUGCA- -5' |
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10782 | 5' | -48.1 | NC_002794.1 | + | 87717 | 0.73 | 0.957439 |
Target: 5'- -cAGCAuaccguacuAGGUAaGCUaCCUACUACGUa -3' miRNA: 3'- cuUCGU---------UCCAUaCGAaGGAUGAUGCA- -5' |
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10782 | 5' | -48.1 | NC_002794.1 | + | 87755 | 1.09 | 0.019494 |
Target: 5'- gGAAGCAAGGUAUGCUUCCUACUACGUa -3' miRNA: 3'- -CUUCGUUCCAUACGAAGGAUGAUGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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