Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10784 | 5' | -51.2 | NC_002794.1 | + | 186533 | 0.66 | 0.996406 |
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Target: 5'- cCUUCUCCA-GGCgGCCUUCc----- -3' miRNA: 3'- aGAAGAGGUaUCGgCGGAAGaucucu -5' |
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| 10784 | 5' | -51.2 | NC_002794.1 | + | 62606 | 0.66 | 0.996406 |
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Target: 5'- -----gCCAccGCCGCCUUC-GGAGAg -3' miRNA: 3'- agaagaGGUauCGGCGGAAGaUCUCU- -5' |
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| 10784 | 5' | -51.2 | NC_002794.1 | + | 150544 | 0.66 | 0.99579 |
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Target: 5'- cCggCgCCAUGGCCGUCUcggcgCUGGAGc -3' miRNA: 3'- aGaaGaGGUAUCGGCGGAa----GAUCUCu -5' |
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| 10784 | 5' | -51.2 | NC_002794.1 | + | 20340 | 0.66 | 0.993409 |
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Target: 5'- cUUUCUCCGgcucAGCCGCUUcgagCUGGAc- -3' miRNA: 3'- aGAAGAGGUa---UCGGCGGAa---GAUCUcu -5' |
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| 10784 | 5' | -51.2 | NC_002794.1 | + | 157184 | 0.67 | 0.990052 |
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Target: 5'- -gUUCUCCGagucGGUCGCCga-UGGAGAc -3' miRNA: 3'- agAAGAGGUa---UCGGCGGaagAUCUCU- -5' |
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| 10784 | 5' | -51.2 | NC_002794.1 | + | 79013 | 0.67 | 0.985485 |
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Target: 5'- ---aCUCCAUcccgcccucGGCCGCCUUCUGc--- -3' miRNA: 3'- agaaGAGGUA---------UCGGCGGAAGAUcucu -5' |
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| 10784 | 5' | -51.2 | NC_002794.1 | + | 18289 | 0.67 | 0.985485 |
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Target: 5'- cCUUCgaaCCG-AGCCGCCcUCUcGAGGg -3' miRNA: 3'- aGAAGa--GGUaUCGGCGGaAGAuCUCU- -5' |
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| 10784 | 5' | -51.2 | NC_002794.1 | + | 146958 | 0.69 | 0.965595 |
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Target: 5'- cUCUcUCUUCAUGGaCCGCCUgUCgcGAGGu -3' miRNA: 3'- -AGA-AGAGGUAUC-GGCGGA-AGauCUCU- -5' |
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| 10784 | 5' | -51.2 | NC_002794.1 | + | 85140 | 1.1 | 0.008679 |
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Target: 5'- gUCUUCUCCAUAGCCGCCUUCUAGAGAc -3' miRNA: 3'- -AGAAGAGGUAUCGGCGGAAGAUCUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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