Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10787 | 5' | -56.8 | NC_002794.1 | + | 52045 | 0.69 | 0.78309 |
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Target: 5'- -----aGGACCUGCUCGAG-AGCUCCg -3' miRNA: 3'- guucccCCUGGACGAGUUCgUUGGGG- -5' |
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| 10787 | 5' | -56.8 | NC_002794.1 | + | 94295 | 0.69 | 0.755337 |
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Target: 5'- --cGGGGGGCg-GCUCGGGC-GCgCCg -3' miRNA: 3'- guuCCCCCUGgaCGAGUUCGuUGgGG- -5' |
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| 10787 | 5' | -56.8 | NC_002794.1 | + | 99460 | 0.69 | 0.755337 |
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Target: 5'- --cGGGGGuAUCggcgGCUCAgagacacggggAGCGACUCCg -3' miRNA: 3'- guuCCCCC-UGGa---CGAGU-----------UCGUUGGGG- -5' |
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| 10787 | 5' | -56.8 | NC_002794.1 | + | 123809 | 0.69 | 0.755337 |
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Target: 5'- uCGGGGGcgcGGACgaGCagGAGCGGCgCCCg -3' miRNA: 3'- -GUUCCC---CCUGgaCGagUUCGUUG-GGG- -5' |
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| 10787 | 5' | -56.8 | NC_002794.1 | + | 178812 | 0.7 | 0.716904 |
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Target: 5'- -cGGGGGGACCggGCacUCGGGUcuCCCg -3' miRNA: 3'- guUCCCCCUGGa-CG--AGUUCGuuGGGg -5' |
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| 10787 | 5' | -56.8 | NC_002794.1 | + | 61110 | 0.7 | 0.716904 |
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Target: 5'- gGAGaaaGGGGACCaGCUCGugcucuGCGcGCCCCu -3' miRNA: 3'- gUUC---CCCCUGGaCGAGUu-----CGU-UGGGG- -5' |
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| 10787 | 5' | -56.8 | NC_002794.1 | + | 134202 | 0.7 | 0.707099 |
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Target: 5'- -uGGuGGGGGCCgucggucGC-CAAGCGACCgCCg -3' miRNA: 3'- guUC-CCCCUGGa------CGaGUUCGUUGG-GG- -5' |
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| 10787 | 5' | -56.8 | NC_002794.1 | + | 183603 | 0.75 | 0.461907 |
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Target: 5'- gGAGGGGGGCCgcgcGCggCcGGCGcCCCCg -3' miRNA: 3'- gUUCCCCCUGGa---CGa-GuUCGUuGGGG- -5' |
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| 10787 | 5' | -56.8 | NC_002794.1 | + | 83467 | 1.11 | 0.002152 |
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Target: 5'- cCAAGGGGGACCUGCUCAAGCAACCCCu -3' miRNA: 3'- -GUUCCCCCUGGACGAGUUCGUUGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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