Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 10787 | 5' | -56.8 | NC_002794.1 | + | 36803 | 0.66 | 0.906272 |
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Target: 5'- cCGAGGucaGGugCUGCgagAGGUAGCCCUc -3' miRNA: 3'- -GUUCCc--CCugGACGag-UUCGUUGGGG- -5' |
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| 10787 | 5' | -56.8 | NC_002794.1 | + | 41353 | 0.66 | 0.905663 |
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Target: 5'- --cGGGGucaccgccgucGGCCUucgcGCUCGcggcgccuccuucGGCGGCCCCg -3' miRNA: 3'- guuCCCC-----------CUGGA----CGAGU-------------UCGUUGGGG- -5' |
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| 10787 | 5' | -56.8 | NC_002794.1 | + | 51262 | 0.66 | 0.906272 |
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Target: 5'- uGGGGGcGGGCCgggagGCagGcGCGGCCCg -3' miRNA: 3'- gUUCCC-CCUGGa----CGagUuCGUUGGGg -5' |
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| 10787 | 5' | -56.8 | NC_002794.1 | + | 145735 | 0.66 | 0.900089 |
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Target: 5'- gGAGGcGGcGgcGCCgGCaUCGacGGCGACCCCg -3' miRNA: 3'- gUUCC-CC-C--UGGaCG-AGU--UCGUUGGGG- -5' |
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| 10787 | 5' | -56.8 | NC_002794.1 | + | 115556 | 0.66 | 0.893683 |
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Target: 5'- --cGGGGGAgUaGC-CGAGCAGCUCg -3' miRNA: 3'- guuCCCCCUgGaCGaGUUCGUUGGGg -5' |
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| 10787 | 5' | -56.8 | NC_002794.1 | + | 52399 | 0.67 | 0.887055 |
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Target: 5'- --cGGucGAcuaCCUGCUCGAGCAGCUCa -3' miRNA: 3'- guuCCccCU---GGACGAGUUCGUUGGGg -5' |
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| 10787 | 5' | -56.8 | NC_002794.1 | + | 123208 | 0.67 | 0.880209 |
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Target: 5'- gGAGauGGACCUGUacaucgucUCGAGCGGCCUg -3' miRNA: 3'- gUUCccCCUGGACG--------AGUUCGUUGGGg -5' |
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| 10787 | 5' | -56.8 | NC_002794.1 | + | 37332 | 0.67 | 0.858413 |
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Target: 5'- --cGGuGGGGCCcggGCggcagCAGGUcgGGCCCCa -3' miRNA: 3'- guuCC-CCCUGGa--CGa----GUUCG--UUGGGG- -5' |
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| 10787 | 5' | -56.8 | NC_002794.1 | + | 83467 | 1.11 | 0.002152 |
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Target: 5'- cCAAGGGGGACCUGCUCAAGCAACCCCu -3' miRNA: 3'- -GUUCCCCCUGGACGAGUUCGUUGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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