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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10788 | 3' | -61.1 | NC_002794.1 | + | 152096 | 0.71 | 0.424837 |
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Target: 5'- cCUGGGauaC-CGCcgUCUGGGGCUuGCUGCg -3' miRNA: 3'- aGACCCg--GuGCG--AGACCUCGG-CGACG- -5' |
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| 10788 | 3' | -61.1 | NC_002794.1 | + | 119389 | 0.71 | 0.424837 |
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Target: 5'- gCUGGGCCA-GCacCUGGcGCCGCaGCc -3' miRNA: 3'- aGACCCGGUgCGa-GACCuCGGCGaCG- -5' |
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| 10788 | 3' | -61.1 | NC_002794.1 | + | 155514 | 0.72 | 0.408094 |
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Target: 5'- --cGGGCCGCcuguuccagucgGgUUUGGAGCUGUUGCa -3' miRNA: 3'- agaCCCGGUG------------CgAGACCUCGGCGACG- -5' |
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| 10788 | 3' | -61.1 | NC_002794.1 | + | 83959 | 0.72 | 0.390967 |
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Target: 5'- cCUGGGCCgccuggaGCGC-CUGGA-CCGCcGCg -3' miRNA: 3'- aGACCCGG-------UGCGaGACCUcGGCGaCG- -5' |
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| 10788 | 3' | -61.1 | NC_002794.1 | + | 12607 | 0.72 | 0.383771 |
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Target: 5'- gCUGcGCCaccaGCGCgUCUGGGGCCGCUucgaGCa -3' miRNA: 3'- aGACcCGG----UGCG-AGACCUCGGCGA----CG- -5' |
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| 10788 | 3' | -61.1 | NC_002794.1 | + | 58627 | 0.72 | 0.375879 |
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Target: 5'- cCUGGugcgccugaucGCCACGCagCUGGgcgacgGGCUGCUGCa -3' miRNA: 3'- aGACC-----------CGGUGCGa-GACC------UCGGCGACG- -5' |
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| 10788 | 3' | -61.1 | NC_002794.1 | + | 58753 | 0.73 | 0.323773 |
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Target: 5'- --cGGGCagCugGCggaGGAGCCGCUGCu -3' miRNA: 3'- agaCCCG--GugCGagaCCUCGGCGACG- -5' |
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| 10788 | 3' | -61.1 | NC_002794.1 | + | 123272 | 0.74 | 0.316784 |
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Target: 5'- gUCcGGGCCGCGgUCgucgcgGGAGaCCuGCUGCu -3' miRNA: 3'- -AGaCCCGGUGCgAGa-----CCUC-GG-CGACG- -5' |
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| 10788 | 3' | -61.1 | NC_002794.1 | + | 91084 | 0.76 | 0.230776 |
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Target: 5'- -gUGGGCUACggGCUCaaGGAGCUGCUGUa -3' miRNA: 3'- agACCCGGUG--CGAGa-CCUCGGCGACG- -5' |
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| 10788 | 3' | -61.1 | NC_002794.1 | + | 81882 | 1.11 | 0.000926 |
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Target: 5'- cUCUGGGCCACGCUCUGGAGCCGCUGCu -3' miRNA: 3'- -AGACCCGGUGCGAGACCUCGGCGACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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