Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10807 | 5' | -51 | NC_002794.1 | + | 146859 | 0.67 | 0.994516 |
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Target: 5'- cGUCGUucGCGuUCGGGUGGggggcgcggucgGCCGaUGAUUa -3' miRNA: 3'- uCAGCA--UGC-AGCCUAUC------------UGGC-ACUAG- -5' |
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| 10807 | 5' | -51 | NC_002794.1 | + | 45548 | 0.69 | 0.982482 |
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Target: 5'- --aCGUAgGUCaGAUAGuCCGUGAUg -3' miRNA: 3'- ucaGCAUgCAGcCUAUCuGGCACUAg -5' |
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| 10807 | 5' | -51 | NC_002794.1 | + | 149775 | 0.7 | 0.973092 |
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Target: 5'- uGUUGgagcggGCGUaCGGGUGGugCGUGgAUCu -3' miRNA: 3'- uCAGCa-----UGCA-GCCUAUCugGCAC-UAG- -5' |
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| 10807 | 5' | -51 | NC_002794.1 | + | 111093 | 0.7 | 0.970262 |
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Target: 5'- gGGUCG-GCGggccCGGcgGGACCGUGGccgUCg -3' miRNA: 3'- -UCAGCaUGCa---GCCuaUCUGGCACU---AG- -5' |
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| 10807 | 5' | -51 | NC_002794.1 | + | 35294 | 0.7 | 0.970262 |
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Target: 5'- cGUCGUGCGcccUCGGAcGGACCGccaguUCa -3' miRNA: 3'- uCAGCAUGC---AGCCUaUCUGGCacu--AG- -5' |
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| 10807 | 5' | -51 | NC_002794.1 | + | 179243 | 0.7 | 0.960507 |
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Target: 5'- aGGUCG-GCGUCGGcgAGACCGc---- -3' miRNA: 3'- -UCAGCaUGCAGCCuaUCUGGCacuag -5' |
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| 10807 | 5' | -51 | NC_002794.1 | + | 132427 | 0.71 | 0.952893 |
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Target: 5'- --gUGUACGUCGGu--GACUGUGGUa -3' miRNA: 3'- ucaGCAUGCAGCCuauCUGGCACUAg -5' |
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| 10807 | 5' | -51 | NC_002794.1 | + | 71484 | 1.1 | 0.010822 |
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Target: 5'- cAGUCGUACGUCGGAUAGACCGUGAUCu -3' miRNA: 3'- -UCAGCAUGCAGCCUAUCUGGCACUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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