miRNA: 3'- uUCGAGAGCUGCA------------UCAG-GUCGUGcga -5'
miRNA: 3'- uuCGaGAGCU-GCAU--CAGGUCGUGCGa -5'
miRNA: 3'- uuCGAGAgCUgCAUCAGGUCGUG---CGA- -5'
miRNA: 3'- uuCGa-GAGCUGCAuCaGGUCGUGCGa -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 10810 | 5' | -55.6 | NC_002794.1 | + | 143187 | 0.69 | 0.807289 |
|
Target: 5'- cGGUUCUCGACGUgcagcuucucgcGGUCcCAGCGCa-- -3' miRNA: 3'- uUCGAGAGCUGCA------------UCAG-GUCGUGcga -5' |
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| 10810 | 5' | -55.6 | NC_002794.1 | + | 179456 | 0.69 | 0.824619 |
|
Target: 5'- -cGC-CUCGGuCGUGauGUUCAGCGCGCa -3' miRNA: 3'- uuCGaGAGCU-GCAU--CAGGUCGUGCGa -5' |
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| 10810 | 5' | -55.6 | NC_002794.1 | + | 182929 | 0.72 | 0.631323 |
|
Target: 5'- -cGCUCUgGAaGUAGUCCGGCGCcggGCUc -3' miRNA: 3'- uuCGAGAgCUgCAUCAGGUCGUG---CGA- -5' |
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| 10810 | 5' | -55.6 | NC_002794.1 | + | 187458 | 0.67 | 0.893134 |
|
Target: 5'- -cGCcgCUCGGCGUcGcUCGGCGCGCg -3' miRNA: 3'- uuCGa-GAGCUGCAuCaGGUCGUGCGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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