miRNA: 3'- -GUCuuGuaGUUgUCGCACCUGUaCUC- -5'
miRNA: 3'- -GUCU-UgUAgUUGUCGCACCUGUaCUC- -5'
miRNA: 3'- gUCUUGua----GUUGUCGCACCUGUa--CUC- -5'
miRNA: 3'- -GUCUUGUAGUUGUCGCACCUGUACUC- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10830 | 3' | -48.7 | NC_002794.1 | + | 149348 | 0.73 | 0.953021 |
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Target: 5'- cCGGggUcgCGGgGGCGUGGACG-GAGu -3' miRNA: 3'- -GUCuuGuaGUUgUCGCACCUGUaCUC- -5' |
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| 10830 | 3' | -48.7 | NC_002794.1 | + | 115268 | 0.73 | 0.934364 |
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Target: 5'- uCAGGcAgGUgAACGGCGUGGACAcGGGg -3' miRNA: 3'- -GUCU-UgUAgUUGUCGCACCUGUaCUC- -5' |
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| 10830 | 3' | -48.7 | NC_002794.1 | + | 23904 | 0.74 | 0.911591 |
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Target: 5'- gGGAGCcgagugCAGCAGCGUGGGCGagcGAGc -3' miRNA: 3'- gUCUUGua----GUUGUCGCACCUGUa--CUC- -5' |
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| 10830 | 3' | -48.7 | NC_002794.1 | + | 59838 | 1.09 | 0.01909 |
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Target: 5'- gCAGAACAUCAACAGCGUGGACAUGAGc -3' miRNA: 3'- -GUCUUGUAGUUGUCGCACCUGUACUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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