Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10841 | 3' | -56.1 | NC_002794.1 | + | 29832 | 0.66 | 0.92527 |
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Target: 5'- ----aGGucCGGGGUCGUGcaGGGGGCAg -3' miRNA: 3'- aaguaCCu-GCUCUAGUAC--CCCCCGUg -5' |
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| 10841 | 3' | -56.1 | NC_002794.1 | + | 126716 | 0.66 | 0.908042 |
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Target: 5'- cUCG-GGGCGAGGgggGUGGGuGGGgACg -3' miRNA: 3'- aAGUaCCUGCUCUag-UACCC-CCCgUG- -5' |
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| 10841 | 3' | -56.1 | NC_002794.1 | + | 25119 | 0.67 | 0.895381 |
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Target: 5'- ----cGGuCGAGAUCGaGcGGGGCACg -3' miRNA: 3'- aaguaCCuGCUCUAGUaCcCCCCGUG- -5' |
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| 10841 | 3' | -56.1 | NC_002794.1 | + | 158086 | 0.67 | 0.888705 |
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Target: 5'- -aCGUGauaaGGgGGGAUCGUGGGGcGGuCGCg -3' miRNA: 3'- aaGUAC----CUgCUCUAGUACCCC-CC-GUG- -5' |
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| 10841 | 3' | -56.1 | NC_002794.1 | + | 116857 | 0.67 | 0.888025 |
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Target: 5'- cUUCAUGcGGCG-GccgugucgcgcucGUCA-GGGGGGCGCc -3' miRNA: 3'- -AAGUAC-CUGCuC-------------UAGUaCCCCCCGUG- -5' |
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| 10841 | 3' | -56.1 | NC_002794.1 | + | 126248 | 0.67 | 0.881803 |
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Target: 5'- aUCGUGGcgcGCGAGGUCGgcggcUGGcuGGcGGCGCu -3' miRNA: 3'- aAGUACC---UGCUCUAGU-----ACC--CC-CCGUG- -5' |
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| 10841 | 3' | -56.1 | NC_002794.1 | + | 146834 | 0.68 | 0.847287 |
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Target: 5'- gUCGuuUGGcGCGAGAgucggugcgcgucguUCgcguucggGUGGGGGGCGCg -3' miRNA: 3'- aAGU--ACC-UGCUCU---------------AG--------UACCCCCCGUG- -5' |
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| 10841 | 3' | -56.1 | NC_002794.1 | + | 9044 | 0.71 | 0.676008 |
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Target: 5'- ---cUGGACGAGAUCAacgaGGcGGuGGCGCa -3' miRNA: 3'- aaguACCUGCUCUAGUa---CC-CC-CCGUG- -5' |
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| 10841 | 3' | -56.1 | NC_002794.1 | + | 51786 | 1.06 | 0.004717 |
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Target: 5'- gUUCAUGGACGAGAUCAUGGGGGGCACc -3' miRNA: 3'- -AAGUACCUGCUCUAGUACCCCCCGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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