Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10843 | 5' | -48.6 | NC_002794.1 | + | 122014 | 0.66 | 0.999397 |
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Target: 5'- aCGucGucuUCUUCGGCGCcgggcgUCUCGUGc -3' miRNA: 3'- cGUuuCuauAGAAGCCGCG------AGAGCAU- -5' |
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| 10843 | 5' | -48.6 | NC_002794.1 | + | 117456 | 0.66 | 0.999397 |
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Target: 5'- cGCgGAAGAcgccgcgGUCgUCGGCGUaCUCGUGg -3' miRNA: 3'- -CG-UUUCUa------UAGaAGCCGCGaGAGCAU- -5' |
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| 10843 | 5' | -48.6 | NC_002794.1 | + | 138323 | 0.66 | 0.999397 |
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Target: 5'- -gGAGGAgGUCUUCGaGCGCUCg---- -3' miRNA: 3'- cgUUUCUaUAGAAGC-CGCGAGagcau -5' |
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| 10843 | 5' | -48.6 | NC_002794.1 | + | 21920 | 0.67 | 0.999084 |
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Target: 5'- gGCGGAGAcgacggCggCGGCGCggcgCUCGUc -3' miRNA: 3'- -CGUUUCUaua---GaaGCCGCGa---GAGCAu -5' |
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| 10843 | 5' | -48.6 | NC_002794.1 | + | 137425 | 0.68 | 0.997197 |
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Target: 5'- cGCGGAGAccgCgcCGGCGCUCUgGa- -3' miRNA: 3'- -CGUUUCUauaGaaGCCGCGAGAgCau -5' |
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| 10843 | 5' | -48.6 | NC_002794.1 | + | 129303 | 0.68 | 0.997197 |
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Target: 5'- cGCGAGGAUAgcaucgCGGUGUUUUCGa- -3' miRNA: 3'- -CGUUUCUAUagaa--GCCGCGAGAGCau -5' |
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| 10843 | 5' | -48.6 | NC_002794.1 | + | 118376 | 0.7 | 0.989037 |
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Target: 5'- gGUAGAGAUAcugccgcUCggCGGCGCUCagccCGUAg -3' miRNA: 3'- -CGUUUCUAU-------AGaaGCCGCGAGa---GCAU- -5' |
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| 10843 | 5' | -48.6 | NC_002794.1 | + | 136158 | 0.83 | 0.48665 |
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Target: 5'- gGCGAAGAggaaGUCUUCGGUGCUCUCc-- -3' miRNA: 3'- -CGUUUCUa---UAGAAGCCGCGAGAGcau -5' |
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| 10843 | 5' | -48.6 | NC_002794.1 | + | 50983 | 1.1 | 0.015663 |
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Target: 5'- cGCAAAGAUAUCUUCGGCGCUCUCGUAc -3' miRNA: 3'- -CGUUUCUAUAGAAGCCGCGAGAGCAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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