Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1085 | 3' | -50.6 | NC_000929.1 | + | 21880 | 0.66 | 0.850269 |
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Target: 5'- uUCCAUgcuGGCaCUGAUGGCagaugcaUUCCUGa -3' miRNA: 3'- -AGGUAauuUCGgGACUACUG-------GAGGAC- -5' |
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| 1085 | 3' | -50.6 | NC_000929.1 | + | 25177 | 0.68 | 0.737478 |
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Target: 5'- gCCAaUAAAGCCCUGAgagcaGGCUgggCCg- -3' miRNA: 3'- aGGUaAUUUCGGGACUa----CUGGa--GGac -5' |
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| 1085 | 3' | -50.6 | NC_000929.1 | + | 28101 | 0.68 | 0.691485 |
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Target: 5'- aCCGUuuaUGAGGCgCUGGUGcguGCCUCCc- -3' miRNA: 3'- aGGUA---AUUUCGgGACUAC---UGGAGGac -5' |
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| 1085 | 3' | -50.6 | NC_000929.1 | + | 34059 | 0.69 | 0.656245 |
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Target: 5'- uUCCAUUAAuGCaauaCUGAauUGGCCUugCCUGa -3' miRNA: 3'- -AGGUAAUUuCGg---GACU--ACUGGA--GGAC- -5' |
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| 1085 | 3' | -50.6 | NC_000929.1 | + | 13414 | 0.74 | 0.370463 |
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Target: 5'- uUCCA-UGAAGCCCUGGacGAaCUCCUGa -3' miRNA: 3'- -AGGUaAUUUCGGGACUa-CUgGAGGAC- -5' |
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| 1085 | 3' | -50.6 | NC_000929.1 | + | 19136 | 1.11 | 0.001206 |
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Target: 5'- aUCCAUUAAAGCCCUGAUGACCUCCUGg -3' miRNA: 3'- -AGGUAAUUUCGGGACUACUGGAGGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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