Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10850 | 5' | -54.1 | NC_002794.1 | + | 24149 | 0.66 | 0.968356 |
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Target: 5'- cUCCgACUCcUGGCCC-GAaACCGGAUa -3' miRNA: 3'- cAGG-UGAGcAUCGGGuCUaUGGUCUA- -5' |
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| 10850 | 5' | -54.1 | NC_002794.1 | + | 56903 | 0.68 | 0.920675 |
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Target: 5'- -gCCGCgCGUGGCCaCGGGcGCCGGGc -3' miRNA: 3'- caGGUGaGCAUCGG-GUCUaUGGUCUa -5' |
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| 10850 | 5' | -54.1 | NC_002794.1 | + | 179218 | 0.68 | 0.920675 |
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Target: 5'- -aCgACggaCGUGGCCCAGAUGCaaCAGGUc -3' miRNA: 3'- caGgUGa--GCAUCGGGUCUAUG--GUCUA- -5' |
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| 10850 | 5' | -54.1 | NC_002794.1 | + | 111800 | 0.69 | 0.867811 |
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Target: 5'- -cCCGCaggUCGUAGCCgCAGAUGucgucCCAGAa -3' miRNA: 3'- caGGUG---AGCAUCGG-GUCUAU-----GGUCUa -5' |
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| 10850 | 5' | -54.1 | NC_002794.1 | + | 80401 | 0.69 | 0.860179 |
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Target: 5'- -cCUACUCGccgcGGCCCAGGUggcGCCGGGc -3' miRNA: 3'- caGGUGAGCa---UCGGGUCUA---UGGUCUa -5' |
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| 10850 | 5' | -54.1 | NC_002794.1 | + | 110865 | 0.71 | 0.77255 |
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Target: 5'- aGUCCACgUCGUccGGCacguucuCCAGGUGCCGGGc -3' miRNA: 3'- -CAGGUG-AGCA--UCG-------GGUCUAUGGUCUa -5' |
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| 10850 | 5' | -54.1 | NC_002794.1 | + | 116998 | 0.77 | 0.462532 |
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Target: 5'- cGUCCGCggCGUcucGCCCAGGUACCAGc- -3' miRNA: 3'- -CAGGUGa-GCAu--CGGGUCUAUGGUCua -5' |
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| 10850 | 5' | -54.1 | NC_002794.1 | + | 45496 | 1.07 | 0.006869 |
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Target: 5'- cGUCCACUCGUAGCCCAGAUACCAGAUg -3' miRNA: 3'- -CAGGUGAGCAUCGGGUCUAUGGUCUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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