Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10858 | 3' | -53.4 | NC_002794.1 | + | 12699 | 0.66 | 0.985604 |
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Target: 5'- uGUUCaugUCGGcCGUGggcUGCGCGUUCCg- -3' miRNA: 3'- gCAAG---AGCUaGUAC---ACGCGCGAGGac -5' |
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| 10858 | 3' | -53.4 | NC_002794.1 | + | 124584 | 0.67 | 0.969507 |
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Target: 5'- uGcgCUCGAUCGUGcGCGUGCUg--- -3' miRNA: 3'- gCaaGAGCUAGUACaCGCGCGAggac -5' |
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| 10858 | 3' | -53.4 | NC_002794.1 | + | 78464 | 0.67 | 0.963166 |
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Target: 5'- gCGgUCUCGGUCAUcagGCGCacgcuCUCCUGu -3' miRNA: 3'- -GCaAGAGCUAGUAca-CGCGc----GAGGAC- -5' |
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| 10858 | 3' | -53.4 | NC_002794.1 | + | 117501 | 0.68 | 0.955959 |
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Target: 5'- gGUcCUCGAUCAggugcuggGUGCGCcggcgcaucuGCUCCa- -3' miRNA: 3'- gCAaGAGCUAGUa-------CACGCG----------CGAGGac -5' |
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| 10858 | 3' | -53.4 | NC_002794.1 | + | 97084 | 0.68 | 0.952018 |
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Target: 5'- uCGcgCggaCGAUCAcGUGCGCGC-CCUa -3' miRNA: 3'- -GCaaGa--GCUAGUaCACGCGCGaGGAc -5' |
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| 10858 | 3' | -53.4 | NC_002794.1 | + | 105646 | 0.7 | 0.872175 |
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Target: 5'- gCGUUCUCcGUCucgccgcgGCGCGCUCCg- -3' miRNA: 3'- -GCAAGAGcUAGuaca----CGCGCGAGGac -5' |
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| 10858 | 3' | -53.4 | NC_002794.1 | + | 35296 | 0.72 | 0.823768 |
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Target: 5'- uCGUgcgccCUCGGacggaccgccaguUCAcccUGUGCGUGCUCCUGu -3' miRNA: 3'- -GCAa----GAGCU-------------AGU---ACACGCGCGAGGAC- -5' |
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| 10858 | 3' | -53.4 | NC_002794.1 | + | 38773 | 1.1 | 0.005946 |
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Target: 5'- aCGUUCUCGAUCAUGUGCGCGCUCCUGa -3' miRNA: 3'- -GCAAGAGCUAGUACACGCGCGAGGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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