Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 1086 | 5' | -50 | NC_000929.1 | + | 21790 | 1.17 | 0.000601 |
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Target: 5'- cGGGCAAAGCGGAAGUAAAGCCAGUGCg -3' miRNA: 3'- -CCCGUUUCGCCUUCAUUUCGGUCACG- -5' |
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| 1086 | 5' | -50 | NC_000929.1 | + | 4719 | 0.72 | 0.529491 |
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Target: 5'- aGGUAAaaGGCGcGAAGguaAAAGCCAGcGCg -3' miRNA: 3'- cCCGUU--UCGC-CUUCa--UUUCGGUCaCG- -5' |
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| 1086 | 5' | -50 | NC_000929.1 | + | 25872 | 0.7 | 0.634615 |
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Target: 5'- aGGcGCAAu-UGGcAGUAcAGCCGGUGCa -3' miRNA: 3'- -CC-CGUUucGCCuUCAUuUCGGUCACG- -5' |
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| 1086 | 5' | -50 | NC_000929.1 | + | 29343 | 0.67 | 0.804146 |
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Target: 5'- uGGCGAcagcGGCGGAAccacAGGGCCGaUGCa -3' miRNA: 3'- cCCGUU----UCGCCUUca--UUUCGGUcACG- -5' |
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| 1086 | 5' | -50 | NC_000929.1 | + | 17351 | 0.66 | 0.852595 |
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Target: 5'- gGGGCAu-GUGGAGGUGAauaAGUgAGUc- -3' miRNA: 3'- -CCCGUuuCGCCUUCAUU---UCGgUCAcg -5' |
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| 1086 | 5' | -50 | NC_000929.1 | + | 25581 | 0.72 | 0.540934 |
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Target: 5'- cGGUAcgccGGGUGGggGUAAuGCCAGUacaggGCg -3' miRNA: 3'- cCCGU----UUCGCCuuCAUUuCGGUCA-----CG- -5' |
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| 1086 | 5' | -50 | NC_000929.1 | + | 3607 | 0.67 | 0.804145 |
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Target: 5'- -aGCGGGGCGGuucAGGUAGuucuGCCAcUGCa -3' miRNA: 3'- ccCGUUUCGCC---UUCAUUu---CGGUcACG- -5' |
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| 1086 | 5' | -50 | NC_000929.1 | + | 22968 | 0.67 | 0.831052 |
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Target: 5'- uGGCAAAGCGGGugccaucacuuGCCAGcuUGUa -3' miRNA: 3'- cCCGUUUCGCCUucauuu-----CGGUC--ACG- -5' |
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| 1086 | 5' | -50 | NC_000929.1 | + | 3865 | 0.67 | 0.833937 |
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Target: 5'- aGGGC---GCGGGAGagcGGCCcuuuacggcguGGUGCg -3' miRNA: 3'- -CCCGuuuCGCCUUCauuUCGG-----------UCACG- -5' |
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| 1086 | 5' | -50 | NC_000929.1 | + | 22690 | 0.66 | 0.852595 |
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Target: 5'- cGGGCAAcgucAGCGucuGGAGUGGAcgcGCCGG-GUc -3' miRNA: 3'- -CCCGUU----UCGC---CUUCAUUU---CGGUCaCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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