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miRNA: 3'- -GUCGaCCCGUUGUa---CGUCU---UCGACGA- -5'
miRNA: 3'- gUCgaCcCGUUgUACGUCUUCGACGa -5'
miRNA: 3'- -GUCGACCCGUUGuaCGucuuCGACGa -5'
miRNA: 3'- -GUCGACC-CguUGUaCGUCuuCGACGa -5'
miRNA: 3'- -GUCGACCCGUUGUACGUCUUCGACGA- -5'
Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio#
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P value |
| Predicted miRNA align pattern | |||||||
| 10867 | 3' | -54.7 | NC_002794.1 | + | 91756 | 0.74 | 0.559096 |
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Target: 5'- cCGGC-GGGCGGCAccgcGCAGGacgGGCUGCUg -3' miRNA: 3'- -GUCGaCCCGUUGUa---CGUCU---UCGACGA- -5' |
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| 10867 | 3' | -54.7 | NC_002794.1 | + | 34221 | 0.74 | 0.538732 |
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Target: 5'- uGGgaGcGCAAgGUGCAGGAGCUGCa -3' miRNA: 3'- gUCgaCcCGUUgUACGUCUUCGACGa -5' |
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| 10867 | 3' | -54.7 | NC_002794.1 | + | 58648 | 0.78 | 0.363306 |
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Target: 5'- gCAGCUGGGCGACggGCu---GCUGCa -3' miRNA: 3'- -GUCGACCCGUUGuaCGucuuCGACGa -5' |
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| 10867 | 3' | -54.7 | NC_002794.1 | + | 117980 | 0.79 | 0.295152 |
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Target: 5'- cCAGCUGGuGguACA-GCAGggGCUGCc -3' miRNA: 3'- -GUCGACC-CguUGUaCGUCuuCGACGa -5' |
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| 10867 | 3' | -54.7 | NC_002794.1 | + | 33486 | 1.08 | 0.004185 |
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Target: 5'- gCAGCUGGGCAACAUGCAGAAGCUGCUg -3' miRNA: 3'- -GUCGACCCGUUGUACGUCUUCGACGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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