Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 10867 | 3' | -54.7 | NC_002794.1 | + | 188273 | 0.66 | 0.932428 |
|
Target: 5'- gGGCcGGGUAGCGgaUGCAGGGcGCcGCg -3' miRNA: 3'- gUCGaCCCGUUGU--ACGUCUU-CGaCGa -5' |
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| 10867 | 3' | -54.7 | NC_002794.1 | + | 189498 | 0.66 | 0.946363 |
|
Target: 5'- cCAGCUGGGCAccucguguccgucGCGgaucaGCGGguGCcGCUc -3' miRNA: 3'- -GUCGACCCGU-------------UGUa----CGUCuuCGaCGA- -5' |
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| 10867 | 3' | -54.7 | NC_002794.1 | + | 190964 | 0.7 | 0.773607 |
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Target: 5'- cCAGCgccucgGGGCAGCAgaGCAGGcAGCgcgGCg -3' miRNA: 3'- -GUCGa-----CCCGUUGUa-CGUCU-UCGa--CGa -5' |
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| 10867 | 3' | -54.7 | NC_002794.1 | + | 192023 | 0.68 | 0.860893 |
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Target: 5'- gCGGCUGcuguuucuguGGCGGCG-GCGGcuGCUGCUg -3' miRNA: 3'- -GUCGAC----------CCGUUGUaCGUCuuCGACGA- -5' |
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| 10867 | 3' | -54.7 | NC_002794.1 | + | 194130 | 0.69 | 0.810602 |
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Target: 5'- aCGGCUGaGGCAGCGgccGCAGcAGCgcccGCg -3' miRNA: 3'- -GUCGAC-CCGUUGUa--CGUCuUCGa---CGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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