miRNA: 3'- guCGACCCGUUGUacGUCUUCGa----CGa -5'
miRNA: 3'- -GUCGaCCCGU---UGu-ACGUCUUCGA-CGa -5'
miRNA: 3'- -GUCGa-CC-CGUUGUA--CGUCUUCgACGA- -5'
miRNA: 3'- gUCGACCcGU---UGUaCGUCuUCGACGA- -5'
miRNA: 3'- gUCG-ACCCGUUGuaCguCuuCG---ACGA- -5'
Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 10867 | 3' | -54.7 | NC_002794.1 | + | 97249 | 0.67 | 0.921605 |
|
Target: 5'- -uGUUGGGCAACAguCGGGAGCacgugGCg -3' miRNA: 3'- guCGACCCGUUGUacGUCUUCGa----CGa -5' |
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| 10867 | 3' | -54.7 | NC_002794.1 | + | 150604 | 0.67 | 0.909791 |
|
Target: 5'- aCGGCcGGGCGccgGCucUGCGGGAGCUcGUg -3' miRNA: 3'- -GUCGaCCCGU---UGu-ACGUCUUCGA-CGa -5' |
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| 10867 | 3' | -54.7 | NC_002794.1 | + | 114563 | 0.67 | 0.909791 |
|
Target: 5'- uCGGCcaGGcGCAGCGUcaGCGGAGGgUGCUc -3' miRNA: 3'- -GUCGa-CC-CGUUGUA--CGUCUUCgACGA- -5' |
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| 10867 | 3' | -54.7 | NC_002794.1 | + | 122556 | 0.67 | 0.921605 |
|
Target: 5'- uGGCUGGaCGagaACGcGCAGcGGCUGCUg -3' miRNA: 3'- gUCGACCcGU---UGUaCGUCuUCGACGA- -5' |
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| 10867 | 3' | -54.7 | NC_002794.1 | + | 102674 | 0.66 | 0.946807 |
|
Target: 5'- gAGCgUGGGCGACcgGguGucGUacgUGCUg -3' miRNA: 3'- gUCG-ACCCGUUGuaCguCuuCG---ACGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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