Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1087 | 5' | -47 | NC_000929.1 | + | 22499 | 0.66 | 0.968727 |
Target: 5'- aCGg--CAAAGccaUCAGCCUGAUUgACCg -3' miRNA: 3'- -GCuuaGUUUCc--GGUCGGACUAAaUGG- -5' |
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1087 | 5' | -47 | NC_000929.1 | + | 292 | 0.67 | 0.956753 |
Target: 5'- aUGAGUCAuuuGGUgaCAGCUUGGUugUUAUCg -3' miRNA: 3'- -GCUUAGUuu-CCG--GUCGGACUA--AAUGG- -5' |
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1087 | 5' | -47 | NC_000929.1 | + | 34949 | 0.67 | 0.947226 |
Target: 5'- ----gUAAuGcGCCagaAGCCUGAUUUACCg -3' miRNA: 3'- gcuuaGUUuC-CGG---UCGGACUAAAUGG- -5' |
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1087 | 5' | -47 | NC_000929.1 | + | 27660 | 0.67 | 0.936408 |
Target: 5'- aCGggUUgcuGAuaccguuccugcGGGCCAGCCg---UUGCCa -3' miRNA: 3'- -GCuuAG---UU------------UCCGGUCGGacuaAAUGG- -5' |
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1087 | 5' | -47 | NC_000929.1 | + | 36206 | 0.68 | 0.924272 |
Target: 5'- uGAAUCAcGGGCCAucagcuacGCgCUGAaagUUAUCa -3' miRNA: 3'- gCUUAGUuUCCGGU--------CG-GACUa--AAUGG- -5' |
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1087 | 5' | -47 | NC_000929.1 | + | 14773 | 0.68 | 0.910814 |
Target: 5'- --uAUCAAAGGCCagucAGCCUG---UGCUg -3' miRNA: 3'- gcuUAGUUUCCGG----UCGGACuaaAUGG- -5' |
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1087 | 5' | -47 | NC_000929.1 | + | 18817 | 0.68 | 0.910106 |
Target: 5'- ----aCAAAGcgccacaGCCAGCCUGAgc-GCCg -3' miRNA: 3'- gcuuaGUUUC-------CGGUCGGACUaaaUGG- -5' |
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1087 | 5' | -47 | NC_000929.1 | + | 10101 | 0.69 | 0.880006 |
Target: 5'- -aGAUCAAccaccgaacgGGGCCAGCgUGAUU--CCa -3' miRNA: 3'- gcUUAGUU----------UCCGGUCGgACUAAauGG- -5' |
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1087 | 5' | -47 | NC_000929.1 | + | 14142 | 0.7 | 0.862745 |
Target: 5'- ----gCAGAGGCCGcccGUCUGAUUUAUg -3' miRNA: 3'- gcuuaGUUUCCGGU---CGGACUAAAUGg -5' |
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1087 | 5' | -47 | NC_000929.1 | + | 26826 | 0.72 | 0.725616 |
Target: 5'- gGGAUCGcuuucugcgaugaAAGGCucugugacCAGCCUGAUUaACCa -3' miRNA: 3'- gCUUAGU-------------UUCCG--------GUCGGACUAAaUGG- -5' |
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1087 | 5' | -47 | NC_000929.1 | + | 24576 | 0.73 | 0.679602 |
Target: 5'- aGGAUCAgGGGGCCaugcacGGCUggGAUUUACCc -3' miRNA: 3'- gCUUAGU-UUCCGG------UCGGa-CUAAAUGG- -5' |
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1087 | 5' | -47 | NC_000929.1 | + | 22107 | 1.15 | 0.001739 |
Target: 5'- cCGAAUCAAAGGCCAGCCUGAUUUACCg -3' miRNA: 3'- -GCUUAGUUUCCGGUCGGACUAAAUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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