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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10878 | 3' | -47.5 | NC_002794.1 | + | 67371 | 0.68 | 0.999385 |
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Target: 5'- cGCCacgUGUC-GAAAGAACGCgUCggCGCc -3' miRNA: 3'- -UGGaa-ACAGuCUUUCUUGCG-AGa-GUG- -5' |
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| 10878 | 3' | -47.5 | NC_002794.1 | + | 83148 | 0.69 | 0.998313 |
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Target: 5'- cGCCUgacgacgaGUCAGcugcGGACGCUCUCGg -3' miRNA: 3'- -UGGAaa------CAGUCuuu-CUUGCGAGAGUg -5' |
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| 10878 | 3' | -47.5 | NC_002794.1 | + | 37237 | 0.69 | 0.998313 |
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Target: 5'- cGCCga--UCAGGucGucGACGCUCUCGCu -3' miRNA: 3'- -UGGaaacAGUCUuuC--UUGCGAGAGUG- -5' |
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| 10878 | 3' | -47.5 | NC_002794.1 | + | 89133 | 0.71 | 0.990179 |
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Target: 5'- gACCguguggUGUCGGAAGGGAuCGCUUgugCAUa -3' miRNA: 3'- -UGGaa----ACAGUCUUUCUU-GCGAGa--GUG- -5' |
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| 10878 | 3' | -47.5 | NC_002794.1 | + | 63033 | 0.75 | 0.929144 |
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Target: 5'- cGCCUgcgcgCAGGAGGAAC-CUCUCACc -3' miRNA: 3'- -UGGAaaca-GUCUUUCUUGcGAGAGUG- -5' |
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| 10878 | 3' | -47.5 | NC_002794.1 | + | 29370 | 1.1 | 0.020069 |
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Target: 5'- gACCUUUGUCAGAAAGAACGCUCUCACc -3' miRNA: 3'- -UGGAAACAGUCUUUCUUGCGAGAGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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