Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10884 | 3' | -48.2 | NC_002794.1 | + | 143013 | 0.66 | 0.999724 |
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Target: 5'- cCGGUCAGcgugCGGUCGGcCGGgUCGAcguGCa -3' miRNA: 3'- aGUCAGUCa---GUCAGUCaGUC-AGUU---UG- -5' |
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| 10884 | 3' | -48.2 | NC_002794.1 | + | 179832 | 0.66 | 0.999651 |
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Target: 5'- --cGUCGGccUCGGUCAGcguggcgCGGUUAAACa -3' miRNA: 3'- aguCAGUC--AGUCAGUCa------GUCAGUUUG- -5' |
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| 10884 | 3' | -48.2 | NC_002794.1 | + | 138060 | 0.67 | 0.999162 |
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Target: 5'- cUUGGcCAGgcaGGUCAGUCGGUUGAAg -3' miRNA: 3'- -AGUCaGUCag-UCAGUCAGUCAGUUUg -5' |
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| 10884 | 3' | -48.2 | NC_002794.1 | + | 176853 | 0.68 | 0.998175 |
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Target: 5'- -aGGUCGGUUAGaUCAGUaCGGagugCGAGCg -3' miRNA: 3'- agUCAGUCAGUC-AGUCA-GUCa---GUUUG- -5' |
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| 10884 | 3' | -48.2 | NC_002794.1 | + | 185184 | 0.72 | 0.973413 |
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Target: 5'- gCGGUCGGcgCGGUCGGcgCGGUCAucGCg -3' miRNA: 3'- aGUCAGUCa-GUCAGUCa-GUCAGUu-UG- -5' |
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| 10884 | 3' | -48.2 | NC_002794.1 | + | 115135 | 0.73 | 0.956547 |
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Target: 5'- -gGGUCGGUCGGguggaGGUCGGUCAc-- -3' miRNA: 3'- agUCAGUCAGUCag---UCAGUCAGUuug -5' |
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| 10884 | 3' | -48.2 | NC_002794.1 | + | 27618 | 0.84 | 0.508732 |
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Target: 5'- cCGGUCGGaCGGcCGGUCGGUCAAGCg -3' miRNA: 3'- aGUCAGUCaGUCaGUCAGUCAGUUUG- -5' |
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| 10884 | 3' | -48.2 | NC_002794.1 | + | 133620 | 0.88 | 0.320043 |
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Target: 5'- gUAGUCAGUCGGUCGGUCGGUgGGAa -3' miRNA: 3'- aGUCAGUCAGUCAGUCAGUCAgUUUg -5' |
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| 10884 | 3' | -48.2 | NC_002794.1 | + | 22745 | 1.12 | 0.01236 |
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Target: 5'- gUCAGUCAGUCAGUCAGUCAGUCAAACg -3' miRNA: 3'- -AGUCAGUCAGUCAGUCAGUCAGUUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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