miRNA: 3'- gaCCuCAUGUUCUGagaCGGCAAGUu -5'
miRNA: 3'- gaCCUCa----------UGUUCUGAGACGGCAAGuu -5'
miRNA: 3'- -GACCUCAUGUUCUGaGACGgCaAGUu -5'
miRNA: 3'- gACCUCAUGUUCUGaGACGGCaaGUU- -5'
miRNA: 3'- -GACCUCAUGUUCUGAGACGGCAAGUU- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10905 | 5' | -51 | NC_002794.1 | + | 100132 | 0.7 | 0.941721 |
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Target: 5'- -cGG-GUACGGGACgcgGCCGUUCGc -3' miRNA: 3'- gaCCuCAUGUUCUGagaCGGCAAGUu -5' |
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| 10905 | 5' | -51 | NC_002794.1 | + | 732 | 0.71 | 0.908554 |
|
Target: 5'- -cGGGGgcucuguuuucGCGGGGCUCUGCCGUUUu- -3' miRNA: 3'- gaCCUCa----------UGUUCUGAGACGGCAAGuu -5' |
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| 10905 | 5' | -51 | NC_002794.1 | + | 172053 | 0.71 | 0.902138 |
|
Target: 5'- aCUGGGGUACGAGAUgCUGUgGcUCAu -3' miRNA: 3'- -GACCUCAUGUUCUGaGACGgCaAGUu -5' |
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| 10905 | 5' | -51 | NC_002794.1 | + | 106898 | 0.79 | 0.561925 |
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Target: 5'- uUGGAGUACGAGGCgCUGCUGcgCGAg -3' miRNA: 3'- gACCUCAUGUUCUGaGACGGCaaGUU- -5' |
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| 10905 | 5' | -51 | NC_002794.1 | + | 8495 | 1.07 | 0.01304 |
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Target: 5'- gCUGGAGUACAAGACUCUGCCGUUCAAg -3' miRNA: 3'- -GACCUCAUGUUCUGAGACGGCAAGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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