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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio![]() |
P value |
Predicted miRNA align pattern | |||||||
10908 | 5' | -51.1 | NC_002794.1 | + | 111921 | 0.67 | 0.991581 |
Target: 5'- -cGAGAGGCUCucGUCCGGGAacAAGa -3' miRNA: 3'- gaCUCUCUGAGu-CGGGUUCUgaUUCa -5' |
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10908 | 5' | -51.1 | NC_002794.1 | + | 9971 | 0.67 | 0.991581 |
Target: 5'- ---cGAGAC-CAGCCCGGGACccAAGc -3' miRNA: 3'- gacuCUCUGaGUCGGGUUCUGa-UUCa -5' |
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10908 | 5' | -51.1 | NC_002794.1 | + | 70404 | 0.67 | 0.98902 |
Target: 5'- -cGGGAGGa--AGCCCAAGAC-GAGa -3' miRNA: 3'- gaCUCUCUgagUCGGGUUCUGaUUCa -5' |
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10908 | 5' | -51.1 | NC_002794.1 | + | 123667 | 0.68 | 0.97763 |
Target: 5'- gCUGGuGucGCUCGGCCCG-GGCUGGGa -3' miRNA: 3'- -GACU-CucUGAGUCGGGUuCUGAUUCa -5' |
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10908 | 5' | -51.1 | NC_002794.1 | + | 6983 | 0.99 | 0.041675 |
Target: 5'- cCUG-GAGACUCAGCCCAAGACUAAGUg -3' miRNA: 3'- -GACuCUCUGAGUCGGGUUCUGAUUCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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