Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 10912 | 3' | -54.5 | NC_002794.1 | + | 144888 | 0.66 | 0.964101 |
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Target: 5'- gCGGGGcgaGACgcucgCGCCGCUGGGccccgccgcCGGCa -3' miRNA: 3'- aGUUCCa--CUGa----GCGGCGACUCu--------GUCG- -5' |
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| 10912 | 3' | -54.5 | NC_002794.1 | + | 189371 | 0.66 | 0.96069 |
|
Target: 5'- cCAGGcUGagccGCUCGCCGCUGuGcuCGGCc -3' miRNA: 3'- aGUUCcAC----UGAGCGGCGACuCu-GUCG- -5' |
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| 10912 | 3' | -54.5 | NC_002794.1 | + | 55972 | 0.66 | 0.96069 |
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Target: 5'- gUCGAGGUGgaugGCguaGCCGCgggcGAGcacCAGCa -3' miRNA: 3'- -AGUUCCAC----UGag-CGGCGa---CUCu--GUCG- -5' |
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| 10912 | 3' | -54.5 | NC_002794.1 | + | 151548 | 0.66 | 0.960337 |
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Target: 5'- cCAAGGagaucGAgUUGCUGCUGAcguagcgccccccGGCGGCg -3' miRNA: 3'- aGUUCCa----CUgAGCGGCGACU-------------CUGUCG- -5' |
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| 10912 | 3' | -54.5 | NC_002794.1 | + | 46747 | 0.66 | 0.957061 |
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Target: 5'- ------cGACUucCGCCGCUGGaucGACGGCg -3' miRNA: 3'- aguuccaCUGA--GCGGCGACU---CUGUCG- -5' |
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| 10912 | 3' | -54.5 | NC_002794.1 | + | 142317 | 0.66 | 0.973083 |
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Target: 5'- -gGAGGcGGCggCGCagGCggcgGAGGCGGCg -3' miRNA: 3'- agUUCCaCUGa-GCGg-CGa---CUCUGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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