Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10912 | 3' | -54.5 | NC_002794.1 | + | 66955 | 0.66 | 0.970292 |
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Target: 5'- cUCGAGGccggcGAgUCGCuCGCcGGGGgAGCg -3' miRNA: 3'- -AGUUCCa----CUgAGCG-GCGaCUCUgUCG- -5' |
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| 10912 | 3' | -54.5 | NC_002794.1 | + | 85260 | 0.66 | 0.972812 |
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Target: 5'- gCAAGGUGGgaagagcacacCUCucggcuccggucaGCgGCUGAGACgaGGCg -3' miRNA: 3'- aGUUCCACU-----------GAG-------------CGgCGACUCUG--UCG- -5' |
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| 10912 | 3' | -54.5 | NC_002794.1 | + | 108142 | 0.66 | 0.973083 |
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Target: 5'- ---cGGcugGAUUCGCCGCcGGGAgcgcCGGCg -3' miRNA: 3'- aguuCCa--CUGAGCGGCGaCUCU----GUCG- -5' |
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| 10912 | 3' | -54.5 | NC_002794.1 | + | 179655 | 0.66 | 0.973083 |
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Target: 5'- gUCAGGcG-GGCUCGCCG----GACGGCa -3' miRNA: 3'- -AGUUC-CaCUGAGCGGCgacuCUGUCG- -5' |
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| 10912 | 3' | -54.5 | NC_002794.1 | + | 138121 | 0.66 | 0.973083 |
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Target: 5'- gCGuGGUG-CUCGUCGCcgGucucGGGCGGCg -3' miRNA: 3'- aGUuCCACuGAGCGGCGa-C----UCUGUCG- -5' |
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| 10912 | 3' | -54.5 | NC_002794.1 | + | 142317 | 0.66 | 0.973083 |
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Target: 5'- -gGAGGcGGCggCGCagGCggcgGAGGCGGCg -3' miRNA: 3'- agUUCCaCUGa-GCGg-CGa---CUCUGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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