Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10918 | 3' | -57.5 | NC_002794.1 | + | 155341 | 0.69 | 0.714368 |
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Target: 5'- gGCCGCCGaCGCGccGAcGGGAGACg- -3' miRNA: 3'- gCGGUGGCcGCGCa-CUaCCUUCUGaa -5' |
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| 10918 | 3' | -57.5 | NC_002794.1 | + | 151966 | 0.69 | 0.714368 |
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Target: 5'- cCGUCGCCGGCGUcucGUGA-GGAGcGGCg- -3' miRNA: 3'- -GCGGUGGCCGCG---CACUaCCUU-CUGaa -5' |
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| 10918 | 3' | -57.5 | NC_002794.1 | + | 58284 | 0.7 | 0.704573 |
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Target: 5'- uCGCCGCCGGgGCcggaGUGcgGGGAGcCg- -3' miRNA: 3'- -GCGGUGGCCgCG----CACuaCCUUCuGaa -5' |
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| 10918 | 3' | -57.5 | NC_002794.1 | + | 182781 | 0.7 | 0.674872 |
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Target: 5'- gCGCgACCGGCGCGaagucccGGggGACg- -3' miRNA: 3'- -GCGgUGGCCGCGCacua---CCuuCUGaa -5' |
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| 10918 | 3' | -57.5 | NC_002794.1 | + | 58195 | 0.7 | 0.668888 |
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Target: 5'- gCGCCGCUGGUGCGgacgcacgguccaguUGAggcGGAAGGCc- -3' miRNA: 3'- -GCGGUGGCCGCGC---------------ACUa--CCUUCUGaa -5' |
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| 10918 | 3' | -57.5 | NC_002794.1 | + | 195600 | 0.71 | 0.644868 |
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Target: 5'- uGCCGCCGcGCGCGcgGAcGcGAGGACg- -3' miRNA: 3'- gCGGUGGC-CGCGCa-CUaC-CUUCUGaa -5' |
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| 10918 | 3' | -57.5 | NC_002794.1 | + | 124561 | 0.71 | 0.60476 |
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Target: 5'- aGCCGCCGGCGCGcuc-GGgcGGCg- -3' miRNA: 3'- gCGGUGGCCGCGCacuaCCuuCUGaa -5' |
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| 10918 | 3' | -57.5 | NC_002794.1 | + | 65200 | 0.73 | 0.539452 |
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Target: 5'- cCGCCGCCGGcCGCGgGcuccgccgcggccucGUGGGAGGCg- -3' miRNA: 3'- -GCGGUGGCC-GCGCaC---------------UACCUUCUGaa -5' |
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| 10918 | 3' | -57.5 | NC_002794.1 | + | 54200 | 0.73 | 0.535561 |
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Target: 5'- gGCCGCCGGCGgGUcGAgcGAAGACg- -3' miRNA: 3'- gCGGUGGCCGCgCA-CUacCUUCUGaa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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