Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 10918 | 3' | -57.5 | NC_002794.1 | + | 133349 | 0.67 | 0.832423 |
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Target: 5'- gCGCCGCCGGCGCcuccgcccaccGUGAc-GAAGAg-- -3' miRNA: 3'- -GCGGUGGCCGCG-----------CACUacCUUCUgaa -5' |
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| 10918 | 3' | -57.5 | NC_002794.1 | + | 151134 | 0.66 | 0.863608 |
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Target: 5'- aCGUCgGCCGGCGCc-GccGGAGGGCUa -3' miRNA: 3'- -GCGG-UGGCCGCGcaCuaCCUUCUGAa -5' |
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| 10918 | 3' | -57.5 | NC_002794.1 | + | 151966 | 0.69 | 0.714368 |
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Target: 5'- cCGUCGCCGGCGUcucGUGA-GGAGcGGCg- -3' miRNA: 3'- -GCGGUGGCCGCG---CACUaCCUU-CUGaa -5' |
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| 10918 | 3' | -57.5 | NC_002794.1 | + | 155341 | 0.69 | 0.714368 |
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Target: 5'- gGCCGCCGaCGCGccGAcGGGAGACg- -3' miRNA: 3'- gCGGUGGCcGCGCa-CUaCCUUCUGaa -5' |
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| 10918 | 3' | -57.5 | NC_002794.1 | + | 160305 | 0.69 | 0.733745 |
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Target: 5'- gCGCuCGCgCGcGCGCGUGGUGGcccAGGCg- -3' miRNA: 3'- -GCG-GUG-GC-CGCGCACUACCu--UCUGaa -5' |
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| 10918 | 3' | -57.5 | NC_002794.1 | + | 165112 | 0.69 | 0.762154 |
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Target: 5'- cCGCgGCUGGCGCGcUGGaggaucUGGAAGcGCUg -3' miRNA: 3'- -GCGgUGGCCGCGC-ACU------ACCUUC-UGAa -5' |
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| 10918 | 3' | -57.5 | NC_002794.1 | + | 182781 | 0.7 | 0.674872 |
|
Target: 5'- gCGCgACCGGCGCGaagucccGGggGACg- -3' miRNA: 3'- -GCGgUGGCCGCGCacua---CCuuCUGaa -5' |
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| 10918 | 3' | -57.5 | NC_002794.1 | + | 193239 | 0.66 | 0.870197 |
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Target: 5'- gGCCGCCGGCGCGcguccccagcaaaUGA-GGA--GCUc -3' miRNA: 3'- gCGGUGGCCGCGC-------------ACUaCCUucUGAa -5' |
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| 10918 | 3' | -57.5 | NC_002794.1 | + | 195600 | 0.71 | 0.644868 |
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Target: 5'- uGCCGCCGcGCGCGcgGAcGcGAGGACg- -3' miRNA: 3'- gCGGUGGC-CGCGCa-CUaC-CUUCUGaa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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