Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 10918 | 3' | -57.5 | NC_002794.1 | + | 46454 | 0.66 | 0.889614 |
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Target: 5'- uCGCCGCgcgugugugccgugUGGUGUGUGugaaggGGAAGGCUc -3' miRNA: 3'- -GCGGUG--------------GCCGCGCACua----CCUUCUGAa -5' |
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| 10918 | 3' | -57.5 | NC_002794.1 | + | 88318 | 0.66 | 0.884916 |
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Target: 5'- gCGCCGgCGGCGaCGaccgcGGUGGggGAg-- -3' miRNA: 3'- -GCGGUgGCCGC-GCa----CUACCuuCUgaa -5' |
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| 10918 | 3' | -57.5 | NC_002794.1 | + | 50943 | 0.66 | 0.884236 |
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Target: 5'- cCGCCGCCGGCaccaaccGCGccaaGGUGGugcuGGACc- -3' miRNA: 3'- -GCGGUGGCCG-------CGCa---CUACCu---UCUGaa -5' |
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| 10918 | 3' | -57.5 | NC_002794.1 | + | 3715 | 0.66 | 0.884916 |
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Target: 5'- uGCUGCCGGCGCGgucGcgGcGGAGcCUg -3' miRNA: 3'- gCGGUGGCCGCGCa--CuaC-CUUCuGAa -5' |
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| 10918 | 3' | -57.5 | NC_002794.1 | + | 56175 | 0.66 | 0.878023 |
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Target: 5'- aGCCGCCGGC-CGUGccGGAcAGGu-- -3' miRNA: 3'- gCGGUGGCCGcGCACuaCCU-UCUgaa -5' |
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| 10918 | 3' | -57.5 | NC_002794.1 | + | 193239 | 0.66 | 0.870197 |
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Target: 5'- gGCCGCCGGCGCGcguccccagcaaaUGA-GGA--GCUc -3' miRNA: 3'- gCGGUGGCCGCGC-------------ACUaCCUucUGAa -5' |
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| 10918 | 3' | -57.5 | NC_002794.1 | + | 88643 | 0.67 | 0.856098 |
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Target: 5'- gCGCCGCCgggcGGCGCGcGAcggGGgcGGCg- -3' miRNA: 3'- -GCGGUGG----CCGCGCaCUa--CCuuCUGaa -5' |
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| 10918 | 3' | -57.5 | NC_002794.1 | + | 73543 | 0.66 | 0.863608 |
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Target: 5'- cCGUCGCCGuGCGCGUGGcguUGGcccuGGCc- -3' miRNA: 3'- -GCGGUGGC-CGCGCACU---ACCuu--CUGaa -5' |
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| 10918 | 3' | -57.5 | NC_002794.1 | + | 124561 | 0.71 | 0.60476 |
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Target: 5'- aGCCGCCGGCGCGcuc-GGgcGGCg- -3' miRNA: 3'- gCGGUGGCCGCGCacuaCCuuCUGaa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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