Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
10919 | 3' | -56.8 | NC_002794.1 | + | 113733 | 0.66 | 0.909425 |
Target: 5'- cUCGGCgUGGGCGUCaCGUgggCGGACa-- -3' miRNA: 3'- -AGUCGgACCCGUAGgGCA---GCUUGacc -5' |
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10919 | 3' | -56.8 | NC_002794.1 | + | 129898 | 0.66 | 0.920206 |
Target: 5'- -gAGCg-GcGGCGUCUccuccggcaccaaCGUCGAACUGGg -3' miRNA: 3'- agUCGgaC-CCGUAGG-------------GCAGCUUGACC- -5' |
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10919 | 3' | -56.8 | NC_002794.1 | + | 157730 | 0.66 | 0.926075 |
Target: 5'- cCAGCUgGGGCGUggaGUCGAGCaGGa -3' miRNA: 3'- aGUCGGaCCCGUAgggCAGCUUGaCC- -5' |
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10919 | 3' | -56.8 | NC_002794.1 | + | 134819 | 0.66 | 0.926075 |
Target: 5'- cCAGaugcuCCUGGGCAUCcCCGUgcaCGGGCa-- -3' miRNA: 3'- aGUC-----GGACCCGUAG-GGCA---GCUUGacc -5' |
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10919 | 3' | -56.8 | NC_002794.1 | + | 155096 | 0.66 | 0.931173 |
Target: 5'- -aGGUgUGGGaggCCuuggCGUCGAGCUGGu -3' miRNA: 3'- agUCGgACCCguaGG----GCAGCUUGACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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