Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10919 | 3' | -56.8 | NC_002794.1 | + | 161774 | 0.7 | 0.74344 |
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Target: 5'- gCGGCC-GGGCAg-CCGUCGGGCggcccGGa -3' miRNA: 3'- aGUCGGaCCCGUagGGCAGCUUGa----CC- -5' |
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| 10919 | 3' | -56.8 | NC_002794.1 | + | 48340 | 0.7 | 0.733949 |
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Target: 5'- cUCGGCCUgGGGCuggUCCGcuUCGAACcGGc -3' miRNA: 3'- -AGUCGGA-CCCGua-GGGC--AGCUUGaCC- -5' |
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| 10919 | 3' | -56.8 | NC_002794.1 | + | 94270 | 0.71 | 0.714725 |
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Target: 5'- gCGGCCggggGGGCGggugccggCCCGcCGGGCgGGg -3' miRNA: 3'- aGUCGGa---CCCGUa-------GGGCaGCUUGaCC- -5' |
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| 10919 | 3' | -56.8 | NC_002794.1 | + | 116966 | 0.71 | 0.699151 |
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Target: 5'- cUCGGCCgGGcGCAacagcacguacuccgUCCCGUCGGGCa-- -3' miRNA: 3'- -AGUCGGaCC-CGU---------------AGGGCAGCUUGacc -5' |
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| 10919 | 3' | -56.8 | NC_002794.1 | + | 49375 | 0.73 | 0.605976 |
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Target: 5'- cUCAGUCUGGag--CCCGUCGAGCUcgcGGa -3' miRNA: 3'- -AGUCGGACCcguaGGGCAGCUUGA---CC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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