Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. |
strand![]() |
Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
10919 | 3' | -56.8 | NC_002794.1 | + | 106378 | 0.67 | 0.897207 |
Target: 5'- cCGGCCUGcGGUGucUCCCGcgaugCGGGCaGGa -3' miRNA: 3'- aGUCGGAC-CCGU--AGGGCa----GCUUGaCC- -5' |
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10919 | 3' | -56.8 | NC_002794.1 | + | 48077 | 0.67 | 0.890769 |
Target: 5'- gCGGCgaccCUGGGCGUCUCG--GAGCUGa -3' miRNA: 3'- aGUCG----GACCCGUAGGGCagCUUGACc -5' |
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10919 | 3' | -56.8 | NC_002794.1 | + | 69410 | 0.68 | 0.831903 |
Target: 5'- cUCGGcCCUGGGUccggCUCGUCGGGaccuuggcCUGGg -3' miRNA: 3'- -AGUC-GGACCCGua--GGGCAGCUU--------GACC- -5' |
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10919 | 3' | -56.8 | NC_002794.1 | + | 19111 | 0.68 | 0.847775 |
Target: 5'- -gGGUCUGcGGCAUCCCcgaacGUCGA--UGGa -3' miRNA: 3'- agUCGGAC-CCGUAGGG-----CAGCUugACC- -5' |
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10919 | 3' | -56.8 | NC_002794.1 | + | 49375 | 0.73 | 0.605976 |
Target: 5'- cUCAGUCUGGag--CCCGUCGAGCUcgcGGa -3' miRNA: 3'- -AGUCGGACCcguaGGGCAGCUUGA---CC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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