Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1092 | 5' | -43.6 | NC_000929.1 | + | 20077 | 0.66 | 0.99844 |
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Target: 5'- uGACGGUUACAGgUAGuCaGCgAcAACCa -3' miRNA: 3'- -CUGUCAAUGUCaAUU-GaCGgUaUUGG- -5' |
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| 1092 | 5' | -43.6 | NC_000929.1 | + | 7970 | 0.66 | 0.998067 |
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Target: 5'- uGACAGUUGCAGUUuuC-GCgGcGGCUu -3' miRNA: 3'- -CUGUCAAUGUCAAuuGaCGgUaUUGG- -5' |
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| 1092 | 5' | -43.6 | NC_000929.1 | + | 25597 | 0.66 | 0.996473 |
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Target: 5'- aGCAGgcuUGCAGgcaguGCUGCCGggcAACUg -3' miRNA: 3'- cUGUCa--AUGUCaau--UGACGGUa--UUGG- -5' |
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| 1092 | 5' | -43.6 | NC_000929.1 | + | 27282 | 0.67 | 0.995746 |
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Target: 5'- uGACGGUaGCAGauguGCUGCU--GGCCc -3' miRNA: 3'- -CUGUCAaUGUCaau-UGACGGuaUUGG- -5' |
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| 1092 | 5' | -43.6 | NC_000929.1 | + | 14194 | 0.67 | 0.995746 |
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Target: 5'- aGACGG--GCGGccu-CUGCCAgaUAACCg -3' miRNA: 3'- -CUGUCaaUGUCaauuGACGGU--AUUGG- -5' |
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| 1092 | 5' | -43.6 | NC_000929.1 | + | 12220 | 0.67 | 0.994901 |
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Target: 5'- cACGGUgacCAGUUGcugcuCUGCCA--GCCa -3' miRNA: 3'- cUGUCAau-GUCAAUu----GACGGUauUGG- -5' |
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| 1092 | 5' | -43.6 | NC_000929.1 | + | 29611 | 0.69 | 0.979684 |
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Target: 5'- gGGCGGUcGCGG-UAACUGUCAccccACCa -3' miRNA: 3'- -CUGUCAaUGUCaAUUGACGGUau--UGG- -5' |
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| 1092 | 5' | -43.6 | NC_000929.1 | + | 14444 | 0.7 | 0.962803 |
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Target: 5'- gGACAGcaUACAGgc-GCUGCCAccacGCCa -3' miRNA: 3'- -CUGUCa-AUGUCaauUGACGGUau--UGG- -5' |
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| 1092 | 5' | -43.6 | NC_000929.1 | + | 27252 | 1.13 | 0.005583 |
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Target: 5'- gGACAGUUACAGUUAACUGCCAUAACCc -3' miRNA: 3'- -CUGUCAAUGUCAAUUGACGGUAUUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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