Results 21 - 23 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
10920 | 5' | -60.2 | NC_002794.1 | + | 78963 | 0.75 | 0.315288 |
Target: 5'- --gGCGCCgUCUCCGGCGGCCggUCcGGc -3' miRNA: 3'- gaaCGCGG-AGAGGUCGCCGG--AGaCCc -5' |
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10920 | 5' | -60.2 | NC_002794.1 | + | 69727 | 0.75 | 0.301693 |
Target: 5'- -cUGCGCCggUUUCUGGcCGGcCCUCUGGGa -3' miRNA: 3'- gaACGCGG--AGAGGUC-GCC-GGAGACCC- -5' |
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10920 | 5' | -60.2 | NC_002794.1 | + | 81038 | 0.76 | 0.282154 |
Target: 5'- --cGCGCgCUCUCCGGCGGCCgagucggCUcGGa -3' miRNA: 3'- gaaCGCG-GAGAGGUCGCCGGa------GAcCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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