miRNA: 3'- gaaCGCGGAGAG-GUCG-------------CCGGagACCC- -5'
miRNA: 3'- gaACGCGGagaGGUCGcCGgAGACCC- -5'
miRNA: 3'- gAACgCGGAGAGGuCGCcGGaGaCCC- -5'
Results 21 - 23 of 23 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 10920 | 5' | -60.2 | NC_002794.1 | + | 183179 | 0.66 | 0.770252 |
|
Target: 5'- --cGCGCUUCUCuCAGCgccuguucaugucGGCCg-UGGGc -3' miRNA: 3'- gaaCGCGGAGAG-GUCG-------------CCGGagACCC- -5' |
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| 10920 | 5' | -60.2 | NC_002794.1 | + | 183252 | 0.69 | 0.637752 |
|
Target: 5'- -cUGCGCCa--CCAGC-GCgUCUGGGg -3' miRNA: 3'- gaACGCGGagaGGUCGcCGgAGACCC- -5' |
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| 10920 | 5' | -60.2 | NC_002794.1 | + | 195148 | 0.66 | 0.780089 |
|
Target: 5'- uUUGgGCCUCUCC-GCGaCCcCgGGGg -3' miRNA: 3'- gAACgCGGAGAGGuCGCcGGaGaCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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