Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
10922 | 5' | -48.4 | NC_002794.1 | + | 180683 | 0.66 | 0.999803 |
Target: 5'- -gGCCGUUCGccggaGACCGGGUGa------ -3' miRNA: 3'- gaUGGCAAGC-----UUGGUCCGCaauaagc -5' |
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10922 | 5' | -48.4 | NC_002794.1 | + | 39801 | 0.66 | 0.999846 |
Target: 5'- -aGCCGUgUCGAAUagagCGGGCGUUGg--- -3' miRNA: 3'- gaUGGCA-AGCUUG----GUCCGCAAUaagc -5' |
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10922 | 5' | -48.4 | NC_002794.1 | + | 39904 | 0.66 | 0.999881 |
Target: 5'- -cGCCGUccccacacgagUCGAA-CGGGCGUg--UCGa -3' miRNA: 3'- gaUGGCA-----------AGCUUgGUCCGCAauaAGC- -5' |
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10922 | 5' | -48.4 | NC_002794.1 | + | 117879 | 0.66 | 0.999908 |
Target: 5'- cCU-CCGccUCGGAacggCGGGCGUUGUUCc -3' miRNA: 3'- -GAuGGCa-AGCUUg---GUCCGCAAUAAGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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