miRNA: 3'- gaUGGCaa-GCUUGGUCCGCaa-UAaGC- -5'
miRNA: 3'- gAUGGCaAGCUUGG-------UCCGCAAUaa-GC- -5'
miRNA: 3'- gaUGGCAAgCUUGGUC--CGCA---AUAAGC- -5'
miRNA: 3'- gaUGGCAAGCuUGgUCCGCAAuaAGC- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10922 | 5' | -48.4 | NC_002794.1 | + | 52452 | 0.7 | 0.992974 |
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Target: 5'- -gACCGgcgCGGGCCGGGCGaagGUcCGg -3' miRNA: 3'- gaUGGCaa-GCUUGGUCCGCaa-UAaGC- -5' |
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| 10922 | 5' | -48.4 | NC_002794.1 | + | 48653 | 0.71 | 0.984475 |
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Target: 5'- uUGCCG-UCGGGCCggggccgGGGCGUUGgcgCGa -3' miRNA: 3'- gAUGGCaAGCUUGG-------UCCGCAAUaa-GC- -5' |
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| 10922 | 5' | -48.4 | NC_002794.1 | + | 105194 | 0.72 | 0.97325 |
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Target: 5'- -cGCCGUUgGGGCCGGccGCGUggcUGUUCGu -3' miRNA: 3'- gaUGGCAAgCUUGGUC--CGCA---AUAAGC- -5' |
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| 10922 | 5' | -48.4 | NC_002794.1 | + | 44019 | 0.75 | 0.911744 |
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Target: 5'- -cGCCGUUCG-GCgAGGCGUUcaUCGg -3' miRNA: 3'- gaUGGCAAGCuUGgUCCGCAAuaAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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