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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio![]() |
P value |
Predicted miRNA align pattern | |||||||
1093 | 5' | -58.6 | NC_000929.1 | + | 34205 | 0.67 | 0.319503 |
Target: 5'- -cCGGCUGGUCUGaCAGGucaGGUuauGUCg -3' miRNA: 3'- uuGCCGACCGGGC-GUCCuugCCA---UAG- -5' |
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1093 | 5' | -58.6 | NC_000929.1 | + | 31194 | 0.69 | 0.253448 |
Target: 5'- gAAUGGCUGGCUgCGCAGu-ACGGUuuuaccGUCa -3' miRNA: 3'- -UUGCCGACCGG-GCGUCcuUGCCA------UAG- -5' |
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1093 | 5' | -58.6 | NC_000929.1 | + | 27588 | 1.07 | 0.000291 |
Target: 5'- cAACGGCUGGCCCGCAGGAACGGUAUCa -3' miRNA: 3'- -UUGCCGACCGGGCGUCCUUGCCAUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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