Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. |
strand![]() |
Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
10961 | 5' | -53.8 | NC_002794.1 | + | 62600 | 0.67 | 0.954074 |
Target: 5'- -gCCAGGGCCACCGccgccuucGGAGA-GCUGc -3' miRNA: 3'- agGGUCUUGGUGGCca------CCUUUaCGAC- -5' |
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10961 | 5' | -53.8 | NC_002794.1 | + | 111503 | 0.67 | 0.950013 |
Target: 5'- -gCCAGGACgagagACCGGagGGAcGUGCUGa -3' miRNA: 3'- agGGUCUUGg----UGGCCa-CCUuUACGAC- -5' |
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10961 | 5' | -53.8 | NC_002794.1 | + | 153286 | 0.68 | 0.94572 |
Target: 5'- aCCCGucGCCcgagacgcagGCCGG-GGAGcgGCUGg -3' miRNA: 3'- aGGGUcuUGG----------UGGCCaCCUUuaCGAC- -5' |
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10961 | 5' | -53.8 | NC_002794.1 | + | 28667 | 0.74 | 0.644931 |
Target: 5'- cCCCGGAaccGCCGCCGGUGGccGucgccgccgccguUGCUGc -3' miRNA: 3'- aGGGUCU---UGGUGGCCACCuuU-------------ACGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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