Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10969 | 5' | -59 | NC_002794.1 | + | 125900 | 0.68 | 0.705009 |
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Target: 5'- cUGGcCGUCCUcGcCUGGC-CGCGACu-- -3' miRNA: 3'- -ACCuGCAGGA-C-GACCGaGCGCUGcuu -5' |
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| 10969 | 5' | -59 | NC_002794.1 | + | 146715 | 0.69 | 0.675545 |
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Target: 5'- cUGGugGcCCUGCUGGauCUCGaucucuGACGAc -3' miRNA: 3'- -ACCugCaGGACGACC--GAGCg-----CUGCUu -5' |
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| 10969 | 5' | -59 | NC_002794.1 | + | 53847 | 0.69 | 0.665645 |
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Target: 5'- cGGGCGUCUcgGCggGGUUCuCGACGAc -3' miRNA: 3'- aCCUGCAGGa-CGa-CCGAGcGCUGCUu -5' |
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| 10969 | 5' | -59 | NC_002794.1 | + | 122646 | 0.7 | 0.625867 |
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Target: 5'- gUGGACGUgaucuaCCUGCUGGCggCGCaGGCc-- -3' miRNA: 3'- -ACCUGCA------GGACGACCGa-GCG-CUGcuu -5' |
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| 10969 | 5' | -59 | NC_002794.1 | + | 80723 | 0.7 | 0.615915 |
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Target: 5'- uUGGACGUCgacCUGGCccucCGCGACGGGg -3' miRNA: 3'- -ACCUGCAGgacGACCGa---GCGCUGCUU- -5' |
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| 10969 | 5' | -59 | NC_002794.1 | + | 128546 | 0.7 | 0.615915 |
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Target: 5'- cGGGugcgcCGUCCUGCUGGUcaUCGCGcuCGGc -3' miRNA: 3'- aCCU-----GCAGGACGACCG--AGCGCu-GCUu -5' |
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| 10969 | 5' | -59 | NC_002794.1 | + | 129087 | 0.7 | 0.596054 |
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Target: 5'- cGGAcCGUCCggugGgUGGaCUgGCGACGAGa -3' miRNA: 3'- aCCU-GCAGGa---CgACC-GAgCGCUGCUU- -5' |
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| 10969 | 5' | -59 | NC_002794.1 | + | 126380 | 0.7 | 0.586158 |
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Target: 5'- gUGGGCGccggggCCUGCgcggcGGCgugCGCGGCGGu -3' miRNA: 3'- -ACCUGCa-----GGACGa----CCGa--GCGCUGCUu -5' |
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| 10969 | 5' | -59 | NC_002794.1 | + | 177973 | 0.7 | 0.586158 |
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Target: 5'- gUGGACGg-UUGgaGGCUCGCGGCa-- -3' miRNA: 3'- -ACCUGCagGACgaCCGAGCGCUGcuu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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