Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 10969 | 5' | -59 | NC_002794.1 | + | 128546 | 0.7 | 0.615915 |
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Target: 5'- cGGGugcgcCGUCCUGCUGGUcaUCGCGcuCGGc -3' miRNA: 3'- aCCU-----GCAGGACGACCG--AGCGCu-GCUu -5' |
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| 10969 | 5' | -59 | NC_002794.1 | + | 129087 | 0.7 | 0.596054 |
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Target: 5'- cGGAcCGUCCggugGgUGGaCUgGCGACGAGa -3' miRNA: 3'- aCCU-GCAGGa---CgACC-GAgCGCUGCUU- -5' |
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| 10969 | 5' | -59 | NC_002794.1 | + | 136109 | 0.68 | 0.724375 |
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Target: 5'- -cGGCGUCCUGCaccUGGCggGCGAaguCGAAg -3' miRNA: 3'- acCUGCAGGACG---ACCGagCGCU---GCUU- -5' |
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| 10969 | 5' | -59 | NC_002794.1 | + | 140092 | 0.66 | 0.798154 |
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Target: 5'- aGGACGUCCa--UGGUcgGCGGCGAu -3' miRNA: 3'- aCCUGCAGGacgACCGagCGCUGCUu -5' |
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| 10969 | 5' | -59 | NC_002794.1 | + | 145782 | 0.68 | 0.714725 |
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Target: 5'- cGGAguCGUCCccgGCcGGCUCGgaCGACGAc -3' miRNA: 3'- aCCU--GCAGGa--CGaCCGAGC--GCUGCUu -5' |
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| 10969 | 5' | -59 | NC_002794.1 | + | 146715 | 0.69 | 0.675545 |
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Target: 5'- cUGGugGcCCUGCUGGauCUCGaucucuGACGAc -3' miRNA: 3'- -ACCugCaGGACGACC--GAGCg-----CUGCUu -5' |
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| 10969 | 5' | -59 | NC_002794.1 | + | 148132 | 0.67 | 0.762137 |
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Target: 5'- cGGGCGcggggccgCCgggGCgagGGCggUCGCGACGGAa -3' miRNA: 3'- aCCUGCa-------GGa--CGa--CCG--AGCGCUGCUU- -5' |
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| 10969 | 5' | -59 | NC_002794.1 | + | 177973 | 0.7 | 0.586158 |
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Target: 5'- gUGGACGg-UUGgaGGCUCGCGGCa-- -3' miRNA: 3'- -ACCUGCagGACgaCCGAGCGCUGcuu -5' |
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| 10969 | 5' | -59 | NC_002794.1 | + | 182933 | 0.67 | 0.789342 |
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Target: 5'- cUGGAaguaGUCCgGCgccgGGCUCGagcCGACGGc -3' miRNA: 3'- -ACCUg---CAGGaCGa---CCGAGC---GCUGCUu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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