Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 10969 | 5' | -59 | NC_002794.1 | + | 91241 | 0.66 | 0.823705 |
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Target: 5'- cGGcCGUCggGCUGcGCUCGCgGACGc- -3' miRNA: 3'- aCCuGCAGgaCGAC-CGAGCG-CUGCuu -5' |
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| 10969 | 5' | -59 | NC_002794.1 | + | 63669 | 0.66 | 0.815343 |
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Target: 5'- cGGACGaCgaGCgcGGC-CGCGGCGAc -3' miRNA: 3'- aCCUGCaGgaCGa-CCGaGCGCUGCUu -5' |
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| 10969 | 5' | -59 | NC_002794.1 | + | 140092 | 0.66 | 0.798154 |
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Target: 5'- aGGACGUCCa--UGGUcgGCGGCGAu -3' miRNA: 3'- aCCUGCAGGacgACCGagCGCUGCUu -5' |
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| 10969 | 5' | -59 | NC_002794.1 | + | 32503 | 0.66 | 0.806823 |
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Target: 5'- -cGACGUCUUGCcGGCUCccgccgugccgGUGAUGAGc -3' miRNA: 3'- acCUGCAGGACGaCCGAG-----------CGCUGCUU- -5' |
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| 10969 | 5' | -59 | NC_002794.1 | + | 182933 | 0.67 | 0.789342 |
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Target: 5'- cUGGAaguaGUCCgGCgccgGGCUCGagcCGACGGc -3' miRNA: 3'- -ACCUg---CAGGaCGa---CCGAGC---GCUGCUu -5' |
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| 10969 | 5' | -59 | NC_002794.1 | + | 105533 | 0.67 | 0.789342 |
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Target: 5'- cGGACGugagaUCCgGCggcGGCUCG-GGCGAAc -3' miRNA: 3'- aCCUGC-----AGGaCGa--CCGAGCgCUGCUU- -5' |
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| 10969 | 5' | -59 | NC_002794.1 | + | 111733 | 0.67 | 0.771325 |
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Target: 5'- cGGGCGaacgcgcucgUCgaGCUGGCgccgCGCGGCGc- -3' miRNA: 3'- aCCUGC----------AGgaCGACCGa---GCGCUGCuu -5' |
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| 10969 | 5' | -59 | NC_002794.1 | + | 86962 | 0.67 | 0.780397 |
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Target: 5'- -cGACGUCC-GCUgcGGCgagcUGCGACGAGg -3' miRNA: 3'- acCUGCAGGaCGA--CCGa---GCGCUGCUU- -5' |
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| 10969 | 5' | -59 | NC_002794.1 | + | 177973 | 0.7 | 0.586158 |
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Target: 5'- gUGGACGg-UUGgaGGCUCGCGGCa-- -3' miRNA: 3'- -ACCUGCagGACgaCCGAGCGCUGcuu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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