miRNA display CGI


Results 1 - 3 of 3 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
1097 3' -53.2 NC_000929.1 + 14394 0.68 0.520694
Target:  5'- cUCUGUAug----GCUGUCAGGGCg- -3'
miRNA:   3'- aAGACGUuaaguaCGGCAGUCCCGaa -5'
1097 3' -53.2 NC_000929.1 + 30913 0.88 0.020669
Target:  5'- uUUCUGCAAUUacUGCCGUCAGGGCUg -3'
miRNA:   3'- -AAGACGUUAAguACGGCAGUCCCGAa -5'
1097 3' -53.2 NC_000929.1 + 31020 0.66 0.636978
Target:  5'- -cCUGCGccagcgCAgauaauuUGCUGUCGGGGCUUu -3'
miRNA:   3'- aaGACGUuaa---GU-------ACGGCAGUCCCGAA- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.