Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10970 | 5' | -51.9 | NC_002794.1 | + | 21997 | 0.69 | 0.948334 |
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Target: 5'- gUCGCCGgcaagccggccaAGGCgUGCga-AACCUGCUCg -3' miRNA: 3'- -AGUGGC------------UCUGaACGuagUUGGACGAG- -5' |
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| 10970 | 5' | -51.9 | NC_002794.1 | + | 77694 | 0.7 | 0.928949 |
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Target: 5'- cUACCGcaGC-UGCAUCAacgcgGCCUGCUCc -3' miRNA: 3'- aGUGGCucUGaACGUAGU-----UGGACGAG- -5' |
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| 10970 | 5' | -51.9 | NC_002794.1 | + | 74628 | 0.7 | 0.911795 |
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Target: 5'- aCACCGuGAUcgGCAaCGACCUGgUCc -3' miRNA: 3'- aGUGGCuCUGaaCGUaGUUGGACgAG- -5' |
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| 10970 | 5' | -51.9 | NC_002794.1 | + | 140549 | 0.71 | 0.899121 |
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Target: 5'- aUCGCCGAGGgacGCGaCGACCUGCg- -3' miRNA: 3'- -AGUGGCUCUgaaCGUaGUUGGACGag -5' |
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| 10970 | 5' | -51.9 | NC_002794.1 | + | 147590 | 0.71 | 0.892419 |
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Target: 5'- aCACCGAGGCgcucGCGgugugCAAUCUGCa- -3' miRNA: 3'- aGUGGCUCUGaa--CGUa----GUUGGACGag -5' |
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| 10970 | 5' | -51.9 | NC_002794.1 | + | 77007 | 0.72 | 0.855428 |
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Target: 5'- gUCACCGGGAUgccgccgGCGcaccUCGACC-GCUCg -3' miRNA: 3'- -AGUGGCUCUGaa-----CGU----AGUUGGaCGAG- -5' |
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| 10970 | 5' | -51.9 | NC_002794.1 | + | 140750 | 0.73 | 0.795023 |
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Target: 5'- aCGCCGAGAagcUGCAcgcCAACCUGCg- -3' miRNA: 3'- aGUGGCUCUga-ACGUa--GUUGGACGag -5' |
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| 10970 | 5' | -51.9 | NC_002794.1 | + | 24194 | 0.73 | 0.795023 |
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Target: 5'- gCACCGAGACcgagcGCggCGGCCcGCUCa -3' miRNA: 3'- aGUGGCUCUGaa---CGuaGUUGGaCGAG- -5' |
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| 10970 | 5' | -51.9 | NC_002794.1 | + | 60010 | 0.75 | 0.71701 |
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Target: 5'- gCGCCGAGc---GCAcCGACCUGCUCg -3' miRNA: 3'- aGUGGCUCugaaCGUaGUUGGACGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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