Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 10970 | 5' | -51.9 | NC_002794.1 | + | 141600 | 0.67 | 0.986273 |
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Target: 5'- gUCGCCGGGcg--GCucggCGACCgGCUCg -3' miRNA: 3'- -AGUGGCUCugaaCGua--GUUGGaCGAG- -5' |
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| 10970 | 5' | -51.9 | NC_002794.1 | + | 8113 | 0.67 | 0.984519 |
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Target: 5'- cCACCGAG-CggGCggCGGCCUcCUCg -3' miRNA: 3'- aGUGGCUCuGaaCGuaGUUGGAcGAG- -5' |
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| 10970 | 5' | -51.9 | NC_002794.1 | + | 127346 | 0.67 | 0.978231 |
|
Target: 5'- -uGCCGAGACgcucgcggcgGCGgcgCGGCC-GCUCg -3' miRNA: 3'- agUGGCUCUGaa--------CGUa--GUUGGaCGAG- -5' |
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| 10970 | 5' | -51.9 | NC_002794.1 | + | 140705 | 0.67 | 0.984519 |
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Target: 5'- cCGCCGcgacccgggaauGGACguuCGUCAACCUGCg- -3' miRNA: 3'- aGUGGC------------UCUGaacGUAGUUGGACGag -5' |
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| 10970 | 5' | -51.9 | NC_002794.1 | + | 10968 | 0.67 | 0.983388 |
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Target: 5'- -gGCCGAGACguccgau-GCCUGCUCc -3' miRNA: 3'- agUGGCUCUGaacguaguUGGACGAG- -5' |
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| 10970 | 5' | -51.9 | NC_002794.1 | + | 191505 | 0.67 | 0.980507 |
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Target: 5'- cCACCGAGAggugccagcgcCggGCGUCGGCCcGCg- -3' miRNA: 3'- aGUGGCUCU-----------GaaCGUAGUUGGaCGag -5' |
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| 10970 | 5' | -51.9 | NC_002794.1 | + | 126384 | 0.67 | 0.978231 |
|
Target: 5'- gCGCCGGGGCcUGCGcggCGGCgUGCg- -3' miRNA: 3'- aGUGGCUCUGaACGUa--GUUGgACGag -5' |
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| 10970 | 5' | -51.9 | NC_002794.1 | + | 125813 | 0.68 | 0.975765 |
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Target: 5'- cUCGCCG-GGCUcUGCGUCcucggcgcCCUGCUg -3' miRNA: 3'- -AGUGGCuCUGA-ACGUAGuu------GGACGAg -5' |
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| 10970 | 5' | -51.9 | NC_002794.1 | + | 60010 | 0.75 | 0.71701 |
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Target: 5'- gCGCCGAGc---GCAcCGACCUGCUCg -3' miRNA: 3'- aGUGGCUCugaaCGUaGUUGGACGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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