Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10975 | 3' | -58.6 | NC_002794.1 | + | 66391 | 0.67 | 0.824696 |
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Target: 5'- cGCGGGC---UCCCCGucGUCCCGc-- -3' miRNA: 3'- -UGCCCGucuAGGGGCucUAGGGCuag -5' |
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| 10975 | 3' | -58.6 | NC_002794.1 | + | 112294 | 0.67 | 0.824696 |
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Target: 5'- uCGGGCGGccCCCCGGG--UCCGAcuUCg -3' miRNA: 3'- uGCCCGUCuaGGGGCUCuaGGGCU--AG- -5' |
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| 10975 | 3' | -58.6 | NC_002794.1 | + | 114805 | 0.67 | 0.832806 |
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Target: 5'- ---cGCAGGUCCCgGAGG-CCCGcgCg -3' miRNA: 3'- ugccCGUCUAGGGgCUCUaGGGCuaG- -5' |
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| 10975 | 3' | -58.6 | NC_002794.1 | + | 4245 | 0.66 | 0.85609 |
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Target: 5'- uCGGuGCGGcUCUCgGuGGUCUCGAUCg -3' miRNA: 3'- uGCC-CGUCuAGGGgCuCUAGGGCUAG- -5' |
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| 10975 | 3' | -58.6 | NC_002794.1 | + | 176958 | 0.66 | 0.85609 |
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Target: 5'- -gGGGCAGcgggacCUCCGGGuAUCCCGGg- -3' miRNA: 3'- ugCCCGUCua----GGGGCUC-UAGGGCUag -5' |
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| 10975 | 3' | -58.6 | NC_002794.1 | + | 86763 | 0.66 | 0.862752 |
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Target: 5'- cCGGGUGGAUCgCCGGGAacgaaccggccgcUCCgGAa- -3' miRNA: 3'- uGCCCGUCUAGgGGCUCU-------------AGGgCUag -5' |
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| 10975 | 3' | -58.6 | NC_002794.1 | + | 33124 | 0.66 | 0.863482 |
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Target: 5'- cGCGGGCAGAcgaUCCaCCaggugcugcGGGA-CCCGAc- -3' miRNA: 3'- -UGCCCGUCU---AGG-GG---------CUCUaGGGCUag -5' |
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| 10975 | 3' | -58.6 | NC_002794.1 | + | 90158 | 0.66 | 0.87068 |
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Target: 5'- uCGGGUccGGGUCCCCGuGAagccUCCGAg- -3' miRNA: 3'- uGCCCG--UCUAGGGGCuCUa---GGGCUag -5' |
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| 10975 | 3' | -58.6 | NC_002794.1 | + | 68839 | 0.66 | 0.877679 |
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Target: 5'- cCGGcGCucgcGGUCCCCGAcGAcCCCGGa- -3' miRNA: 3'- uGCC-CGu---CUAGGGGCU-CUaGGGCUag -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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